Organoid Co-culture Methods to Capture Cancer Cell-Natural Killer Cell Interactions

Isaac S Chan; Andrew J Ewald

doi:10.1007/978-1-0716-2160-8_17

Organoid Co-culture Methods to Capture Cancer Cell-Natural Killer Cell Interactions

Methods Mol Biol. 2022:2463:235-250. doi: 10.1007/978-1-0716-2160-8_17.

Authors

Isaac S Chan^{1

2}, Andrew J Ewald^{3

4}

Affiliations

¹ Division of Hematology and Oncology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. isaac.chan@utsouthwestern.edu.
² Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA. isaac.chan@utsouthwestern.edu.
³ Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. andrew.ewald@jhmi.edu.
⁴ Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA. andrew.ewald@jhmi.edu.

PMID: 35344179
DOI: 10.1007/978-1-0716-2160-8_17

Abstract

Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.

Keywords: 3D culture; Antibody-dependent cell-mediated cytotoxicity; Breast cancer; Cancer metastasis; Immune escape; Innate immune system; Natural killer cells; Organoids; Organotypic co-culture; Tumor immunosurveillance.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Coculture Techniques
Humans
Killer Cells, Natural
Neoplasms*
Organoids*

Abstract

Publication types

MeSH terms

Grants and funding