Azadirachta indica-wrapped copper oxide nanoparticles as a novel functional material in cardiomyocyte cells: An ecotoxicity assessment on the embryonic development of Danio rerio

Abstract

This research reports on the production of copper oxide nanoparticles (CuO NPs) through the green synthesis method using Azadirachta indica (Ai) flower extract. Synthesized Ai-CuO NPs are characterized by Zeta Potential, TGA, SEM and TEM analysis. The Ai-CuO NPs gave a maximum peak at 270 nm. As per XRD studies, the Ai-CuO NPs obtained were crystalline. FTIR spectrum Ai-CuO NPs showed the presence of functional groups like the O-H group, aromatic group, etc. TEM and SEM assist in investigating the size and morphology of the Ai-CuO NPs, which were spherical and varied in size between 10.11 nm and 17.54 nm. EDAX showed that Ai-CuO NPs were pure with no impurities. The synthesized Ai-CuO NPs were then analyzed for their cytotoxicity at various concentrations (5, 10, 20, 30, 40 and 50 μg/mL) against H9c2 cardiomyocyte cells using MTT assay. DOX-induced H9c2 cell damage of apoptosis and ROS. The nanoparticle formed by Ai-CuO was cured with different concentrations (5, 10 and 20 μg/mL). In zebrafish, 48 hpf and 72 hpf were measured at 75 μM to reduce dysfunction and mortality during organ development. These results can have a beneficial impact on eco-toxicological effects.

Keywords: Apoptosis; CuO nanoparticles; H9c2 cell; Neem; Zebrafish embryos.