Measuring Endogenous GA and IAA

Yunping Chen; Zhiwu Dan; Shaoqing Li

doi:10.21769/BioProtoc.4332

Measuring Endogenous GA and IAA

Bio Protoc. 2022 Feb 20;12(4):e4332. doi: 10.21769/BioProtoc.4332.

Authors

Yunping Chen¹, Zhiwu Dan¹, Shaoqing Li¹

Affiliation

¹ State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China.

Abstract

Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system. This protocol describes a detailed procedure to extract and quantify indole-3-acetic acid (IAA) and gibberellin acid (GA) in rice (Oryza sativa) tissues using high-performance liquid chromatography (HPLC)-based method.

Keywords: GA; HPLC; IAA; Oryza sativa; Phytohormones.