Polyethylene, polystyrene and lignocellulose wastes as mealworm (Tenebrio molitor L.) diets and their impact on the breeding condition, biometric parameters, metabolism, and digestive microbiome

Abstract

This study aimed to identify the extent to which a diet of oatmeal and polymers affects the development of mealworms, their microbiome, the biochemical activity of their digestive system, and their feed-metabolizing capacity. With a polystyrene diet, feed loss was most significant, as indicated by FTIR (Fourier-transform infrared spectroscopy) of frass, which showed that polystyrene was the only compound that was chemically modified. Compared to the control diet, mealworm larvae developed best on polyethylene regranulate (PE-reg), quickly transiting from one developmental stage to another with minor mass loss. A lignocellulose-based diet was the least beneficial for mealworm development. A polystyrene diet was most beneficial in terms of the protein content in larvae, but the contents and quality (usefulness as food) of fatty acids in the insects fed these wastes were significantly lower than in the control insects. For each diet, specific microbial cultures formed, and the presence of protozoa and various biochemical activities suggested different survival strategies and assimilation mechanisms facilitating survival. Despite profound changes in the microbiota and biochemistry of the digestive tract of mealworms fed waste-based diets, this study indicates their potential for utilizing PE-reg and polystyrene.

Keywords: Animal welfare; Biochemical properties; Microbiome modification; Nitrogen cycle; Waste bioconversion.