Silicone polyether surfactant enhances bacterial cellulose synthesis and water holding capacity

Magdalena Szymańska; Jakub Hoppe; Michał Dutkiewicz; Peter Sobolewski; Magdalena Palacz; Ewa Janus; Beata Zielińska; Radosław Drozd

doi:10.1016/j.ijbiomac.2022.03.124

Silicone polyether surfactant enhances bacterial cellulose synthesis and water holding capacity

Int J Biol Macromol. 2022 May 31:208:642-653. doi: 10.1016/j.ijbiomac.2022.03.124. Epub 2022 Mar 22.

Authors

Magdalena Szymańska¹, Jakub Hoppe², Michał Dutkiewicz³, Peter Sobolewski⁴, Magdalena Palacz³, Ewa Janus⁵, Beata Zielińska⁶, Radosław Drozd⁷

Affiliations

¹ Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, 45 Piastów Avenue, 70-311 Szczecin, Poland.
² Faculty of Chemistry, Adam Mickiewicz University, 89b Umultowska Str., 61-614 Poznań Poland.
³ Poznań Science and Technology Park, Adam Mickiewicz University Foundation, 46 Rubież Str., 61-612 Poznań, Poland.
⁴ Department of Polymer and Biomaterials Science, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin, 45 Piastów Avenue, 71-311 Szczecin, Poland.
⁵ Department of Chemical Organic Technology, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, 10 Pulawskiego Str., 70-322 Szczecin, Poland.
⁶ Department of Nanomaterials Physicochemistry, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, 42 Piastów Avenue, 71-065 Szczecin, Poland.
⁷ Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, 45 Piastów Avenue, 70-311 Szczecin, Poland. Electronic address: rdrozd@zut.edu.pl.

PMID: 35337915
DOI: 10.1016/j.ijbiomac.2022.03.124

Abstract

The versatility and unique properties of bacterial cellulose (BC) motivate research into enhancing its synthesis. Here a silicone polyether surfactant (SPS) was synthesized and tested as a non-nutritional additive to the cultivation media of Komagataeibacter xylinus. The addition of SPS to the Hestrin-Schramm (HS) medium resulted in a concentration-dependent decrease in surface tension from 59.57 ± 0.37 mN/m to 30.05 ± 0.41 mN/m (for 0.1% addition) that was correlated with an increased yield of BC, up to 37% wet mass for surfactant concentration close to its critical micelle concentration (0.008%). Physicochemical characterization of bacterial cellulose obtained in presence of SPS, showed that surfactant is not incorporated into BC structure and has a moderate effect on its crystallinity, thermal stability. Moreover, the water holding capacity was enhanced by over 40%. Importantly, obtained BC did not affect L929 murine fibroblast cell viability. We conclude that SPS provides an eco-friendly approach to increasing BC yield in static culture, enabling more widespread industrial and biomedical applications.

Keywords: Bacterial cellulose; Cytotoxicity; Silicone polyether surfactants; Yield.

MeSH terms

Animals
Bacteria
Cellulose / chemistry
Culture Media / chemistry
Gluconacetobacter xylinus*
Mice
Silicones
Surface-Active Agents* / pharmacology
Water

Substances

Culture Media
Silicones
Surface-Active Agents
Water
Cellulose