Preparation of Low-Molecular-Weight Fucoidan with Anticoagulant Activity by Photocatalytic Degradation Method

Yihui Qi; Lilong Wang; Ying You; Xiaona Sun; Chengrong Wen; Yinghuan Fu; Shuang Song

doi:10.3390/foods11060822

Preparation of Low-Molecular-Weight Fucoidan with Anticoagulant Activity by Photocatalytic Degradation Method

Foods. 2022 Mar 13;11(6):822. doi: 10.3390/foods11060822.

Authors

Yihui Qi^{1

2}, Lilong Wang^{1

2}, Ying You^{1

2

3}, Xiaona Sun^{1

2}, Chengrong Wen^{1

2}, Yinghuan Fu^{1

2

4}, Shuang Song^{1

2}

Affiliations

¹ National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
² National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China.
³ College of Food Science and Engineering, Jilin Agricultural University, Changchun 130117, China.
⁴ School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China.

Abstract

It is a challenge to degrade sulfated polysaccharides without stripping sulfate groups. In the present study, a photocatalytic method was applied to degrade fucoidan, a sulfated polysaccharide from brown algae. The degradation with varying addition amounts of H₂O₂ and TiO₂ were monitored by high performance gel permeation chromatography (HPGPC) and thin layer chromatography (TLC), and fucoidan was efficiently degraded with 5% TiO₂ and 0.95% H₂O₂. A comparison of the chemical compositions of 2 products obtained after 0.5 h and 3 h illumination, DF-0.5 (average Mw 90 kDa) and DF-3 (average Mw 3 kDa), respectively, with those of fucoidan indicates the photocatalytic degradation did not strip the sulfate groups, but reduced the galactose/fucose ratio. Moreover, 12 oligosaccharides in DF-3 were identified by HPLC-ESI-MSⁿ and 10 of them were sulfated. In addition, DF-0.5 showed anticoagulant activity as strong as fucoidan while DF-3 could specifically prolong the activated partial thromboplastin time. All samples exerted inhibition effects on the intrinsic pathway FXII in a dose-dependent manner. Thus, photocatalytic degradation demonstrated the potential to prepare sulfated low-molecular-weight fucoidan with anticoagulant activity.

Keywords: anticoagulant activity; fucoidan; photocatalytic degradation.

Abstract

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