From a clinical point of view, knowledge of the commensal microbial flora of the conjunctival sac in healthy individuals proves to be of great importance. The aim of this study was to assess the presence and the composition of normal ocular microflora of healthy donkeys. Fourteen clinically healthy donkeys (Equus asinus) participated in the study. After prior ophthalmological examination, which showed no abnormalities, a conjunctival swab was taken from each donkey from the right and left eye. Species-specific identification was based on a morphological assessment of bacterial colonies stained with the Gram technique, as well as on biochemical properties and the disk-diffusion method. Around 82% of samples were positive for bacteria cultivation; Pantoea agglomerans was the most prevalently detected species, followed by Moraxella lacunata. In conclusion, our study made it possible to determine the commensal flora of the conjunctival sac in donkeys. The obtained results also showed discrepancies in the composition of the conjunctival sac flora of donkeys and horses, despite the geographical proximity of performed studies. Knowledge of the commensal conjunctival flora of donkeys is of great clinical importance due to their greater exposure to corneal damage and infections than horses.
Keywords: Central Europe; commensals; conjunctival flora; donkeys.