Plant cell cultures: An enzymatic tool for polyphenolic and flavonoid transformations

Tarik A Mohamed; Sherin K Ali; Abdelsamed I Elshamy; Ibrahim A Saleh; Mahmoud A A Ibrahim; Mohamed A M Atia; Shifaa O Alshammari; Abou El-Hamd H Mohamed; Taha A Hussien; Ahmed R Hamed; Hesham R El Saedi; Nahla S Abdel-Azim; Khaled A Shams; Thomas Efferth; Mahmoud Saker; Paul W Paré; Mohamed-Elamir F Hegazy

doi:10.1016/j.phymed.2022.154019

Plant cell cultures: An enzymatic tool for polyphenolic and flavonoid transformations

Phytomedicine. 2022 Jun:100:154019. doi: 10.1016/j.phymed.2022.154019. Epub 2022 Feb 27.

Authors

Tarik A Mohamed¹, Sherin K Ali¹, Abdelsamed I Elshamy², Ibrahim A Saleh¹, Mahmoud A A Ibrahim³, Mohamed A M Atia⁴, Shifaa O Alshammari⁵, Abou El-Hamd H Mohamed⁶, Taha A Hussien⁷, Ahmed R Hamed¹, Hesham R El Saedi⁸, Nahla S Abdel-Azim¹, Khaled A Shams¹, Thomas Efferth⁹, Mahmoud Saker¹⁰, Paul W Paré¹¹, Mohamed-Elamir F Hegazy¹²

Affiliations

¹ Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.
² Natural Compounds Chemistry Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.
³ Computational Chemistry Laboratory, Chemistry Department, Faculty of Science, Minia University, Minia 61519, Egypt.
⁴ Molecular Genetics and Genome Mapping Laboratory, Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza 12619, Egypt.
⁵ Department of Biology, College of Science, University of Hafr Al Batin, Hafar Al Batin, Saudi Arabia.
⁶ Department of Chemistry, Faculty of Science, Aswan University, Aswan 81528, Egypt.
⁷ Pharmacognosy Department, Faculty of Pharmacy, Sphinx University, New Assiut City, Assiut 10, Egypt.
⁸ Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm S-10691, Sweden; nternational Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China; Department of Chemistry, Faculty of Science, El-Menoufia University, Shebin El-Kom 32512, Egypt.
⁹ Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Staudinger Weg 5, Mainz 55128, Germany. Electronic address: efferth@uni-mainz.de.
¹⁰ Genetic Engineering and Biotech. Division, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.
¹¹ Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
¹² Chemistry of Medicinal Plants Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt. Electronic address: mohegazy@uni-mainz.de.

PMID: 35325826
DOI: 10.1016/j.phymed.2022.154019

Abstract

Background: In the pharmaceutical sector, tissue culture techniques for large-scale production of natural chemicals can be a less expensive alternative to large-scale synthesis. Although recent biotransformation research have used plant cell cultures to target a wide range of bioactive compounds, more compiled information and synopses are needed to better understand metabolic pathways and improve biotransformation efficiencies.

Purpose: This report reviews the biochemical transformation of phenolic natural products by plant cell cultures in order to identify potential novel biotechnological approaches for ensuring more homogeneous and stable phenolic production year-round under controlled environmental conditions.

Methods: Articles on the use of plant cell culture for polyphenolic and flavonoid transformations (1988 - 2021) were retrieved from SciFinder, PubMed, Scopus, and Web of Science through electronic and manual search in English. Following that, the authors chose the required papers based on the criteria they defined. The following keywords were used for the online search: biotransformation, Plant cell cultures, flavonoids, phenolics, and pharmaceutical products.

Results: The initial search found a total of 96 articles. However, only 70 of them were selected as they met the inclusion criteria defined by the authors. The analysis of these studies revealed that plant tissue culture is applicable for the large-scale production of plant secondary metabolites including the phenolics, which have high therapeutic value.

Conclusion: Plant tissue cultures could be employed as an efficient technique for producing secondary metabolites including phenolics. Phenolics possess a wide range of therapeutic benefits, as anti-oxidant, anti-cancer, and anti-inflammatory properties. Callus culture, suspension cultures, transformation, and other procedures have been used to improve the synthesis of phenolics. Their production on a large scale is now achievable. More breakthroughs will lead to newer insights and, without a doubt, to a new era of phenolics-based pharmacological agents for the treatment of a variety of infectious and degenerative disorders.

Keywords: Enzymatic biotransformation; Flavonoid; Pharmaceutical products; Plant cell cultures; Polyphenolic.

MeSH terms

Antioxidants / metabolism
Cell Culture Techniques
Flavonoids* / analysis
Phenols* / analysis
Plants / metabolism

Substances

Antioxidants
Flavonoids
Phenols