Do 2 H and 18 O in leaf water reflect environmental drivers differently?

Abstract

We compiled hydrogen and oxygen stable isotope compositions (δ² H and δ¹⁸ O) of leaf water from multiple biomes to examine variations with environmental drivers. Leaf water δ² H was more closely correlated with δ² H of xylem water or atmospheric vapour, whereas leaf water δ¹⁸ O was more closely correlated with air relative humidity. This resulted from the larger proportional range for δ² H of meteoric waters relative to the extent of leaf water evaporative enrichment compared with δ¹⁸ O. We next expressed leaf water as isotopic enrichment above xylem water (Δ² H and Δ¹⁸ O) to remove the impact of xylem water isotopic variation. For Δ² H, leaf water still correlated with atmospheric vapour, whereas Δ¹⁸ O showed no such correlation. This was explained by covariance between air relative humidity and the Δ¹⁸ O of atmospheric vapour. This is consistent with a previously observed diurnal correlation between air relative humidity and the deuterium excess of atmospheric vapour across a range of ecosystems. We conclude that ² H and ¹⁸ O in leaf water do indeed reflect the balance of environmental drivers differently; our results have implications for understanding isotopic effects associated with water cycling in terrestrial ecosystems and for inferring environmental change from isotopic biomarkers that act as proxies for leaf water.

Keywords: evaporative enrichment; isotopic biomarker; leaf water; relative humidity; stable isotopes.