The mRNA and miRNA profiles of goat bronchial epithelial cells stimulated by Pasteurella multocida strains of serotype A and D

Qi An; Si Chen; Luyin Zhang; Zhenxing Zhang; Yiwen Cheng; Haotian Wu; Ang Liu; Zhen Chen; Bin Li; Jie Chen; Yiying Zheng; Churiga Man; Fengyang Wang; Qiaoling Chen; Li Du

doi:10.7717/peerj.13047

The mRNA and miRNA profiles of goat bronchial epithelial cells stimulated by Pasteurella multocida strains of serotype A and D

PeerJ. 2022 Mar 18:10:e13047. doi: 10.7717/peerj.13047. eCollection 2022.

Authors

Qi An¹, Si Chen¹, Luyin Zhang¹, Zhenxing Zhang¹, Yiwen Cheng¹, Haotian Wu¹, Ang Liu¹, Zhen Chen¹, Bin Li¹, Jie Chen¹, Yiying Zheng¹, Churiga Man¹, Fengyang Wang¹, Qiaoling Chen¹, Li Du¹

Affiliation

¹ Hainan Key Lab of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Haikou, Hainan, China.

Abstract

Pasteurella multocida (P. multocida) is a zoonotic bacterium that predominantly colonizes the respiratory tract and lungs of a variety of farmed and wild animals, and causes severe respiratory disease. To investigate the characteristics of the host immune response induced by P. multocida strains of serotype A and D, high-throughput mRNA-Seq and miRNA-Seq were performed to analyze the changes in goat bronchial epithelial cells stimulated by these two serotypes of P. multocida for 4 h. Quantitative RT-PCR was used to validate the randomly selected genes and miRNAs. The results revealed 204 and 117 differentially expressed mRNAs (|log₂(Fold-change)| ≥ 1, p-value < 0.05) in the P. multocida serotype A and D stimulated groups, respectively. Meanwhile, the number of differentially expressed miRNAs (|log₂(Fold-change)| > 0.1, p-value < 0.05) were 269 and 290, respectively. GO and KEGG enrichment analyses revealed 13 GO terms (p-value < 0.05) and four KEGG pathways (p-value < 0.05) associated with immunity. In the serotype A-stimulated group, the immune-related pathways were the GABAergic synapse and Toll-like receptor signaling pathways, while in the serotype D-stimulated group, the immune-related pathways were the phagosome and B cell receptor signaling pathways. Based on the predicted results of TargetScan and miRanda, the differentially expressed mRNA-miRNA network of immune-related GO terms and KEGG pathways was constructed. According to the cell morphological changes and the significant immune-related KEGG pathways, it was speculated that the P. multocida serotype D strain-stimulated goat bronchial epithelial cells may induce a cellular immune response earlier than serotype A-stimulated cells. Our study provides valuable insight into the host immune response mechanism induced by P. multocida strains of serotype A and D.

Keywords: Goat bronchial epithelial cells; Immune response; Pasteurella multocida; mRNA-Seq; miRNA-Seq.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Epithelial Cells
Goats / genetics
Lung / microbiology
MicroRNAs* / genetics
Pasteurella multocida* / genetics
RNA, Messenger / genetics
Serogroup

Substances

RNA, Messenger
MicroRNAs

Grants and funding

This study was supported by Academician Innovation Platform Project of Hainan Province (No. YSPTZX202013), China Agriculture Research System of MOF and MARA (CARS-38), Academician Group Innovation Centre of Hainan Province. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.