Lipid Extraction and Analysis

Henry H Harrison; Jennifer L Watts

doi:10.1007/978-1-0716-2181-3_14

Lipid Extraction and Analysis

Methods Mol Biol. 2022:2468:271-281. doi: 10.1007/978-1-0716-2181-3_14.

Authors

Henry H Harrison¹, Jennifer L Watts²

Affiliations

¹ School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
² School of Molecular Biosciences, Washington State University, Pullman, WA, USA. jwatts@wsu.edu.

PMID: 35320570
DOI: 10.1007/978-1-0716-2181-3_14

Abstract

Lipids are major components of cellular membranes and energy stores. Lipids contribute vital structural, energetic, and signaling functions. We have optimized methods to extract and analyze lipids from the nematode Caenorhabditis elegans based on standard methods. Here we describe a method to extract total lipids from C. elegans larvae, adults, or embryos. We describe a thin-layer chromatography method to separate major lipid classes and a gas chromatography method to analyze fatty acid composition from lipid extracts, lipid fractions, or directly from nematode larvae, adults, or embryos.

Keywords: Fatty acid methyl esters; Gas chromatography; Lipid extraction; Neutral lipids; Phospholipids; Thin-layer chromatography.

MeSH terms

Animals
Caenorhabditis elegans*
Cell Membrane / chemistry
Chromatography, Gas / methods
Chromatography, Thin Layer / methods
Fatty Acids* / analysis

Substances

Fatty Acids

Grants and funding

R01 GM133883/GM/NIGMS NIH HHS/United States