Bimetallic gold-platinum nanoparticles as a drug delivery system coated with a new drug to target glioblastoma

Anastasia P Stavropoulou; Maria Theodosiou; Elias Sakellis; Nikos Boukos; Giorgos Papanastasiou; Chengjia Wang; Adriana Tavares; Carlos Alcaide Corral; Dimitrios Gournis; Nikolaos Chalmpes; Oliviero L Gobbo; Eleni K Efthimiadou

doi:10.1016/j.colsurfb.2022.112463

Bimetallic gold-platinum nanoparticles as a drug delivery system coated with a new drug to target glioblastoma

Colloids Surf B Biointerfaces. 2022 Jun:214:112463. doi: 10.1016/j.colsurfb.2022.112463. Epub 2022 Mar 16.

Affiliations

¹ Inorganic Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimioupolis, Zografou, Athens, Greece; Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Agia Paraskevi, Athens, Greece.
² Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Agia Paraskevi, Athens, Greece.
³ School of Computer Science and Electronic Engineering, University of Essex, Colchester Campus, CO4 3SQ, UK; Edinburgh Imaging Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH16 4TJ, UK.
⁴ Edinburgh Imaging Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH16 4TJ, UK.
⁵ Department of Materials Science & Engineering, University of Ioannina, 45110 Ioannina, Greece.
⁶ Trinity College Dublin, School of Pharmacy & Pharmaceutical Sciences, Dublin, Ireland.
⁷ Inorganic Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimioupolis, Zografou, Athens, Greece; Institute of Nanoscience and Nanotechnology, NCSR "Demokritos", Agia Paraskevi, Athens, Greece. Electronic address: efthim@chem.uoa.gr.

PMID: 35316703
DOI: 10.1016/j.colsurfb.2022.112463

Abstract

A drug delivery nanosystem of noble bimetallic nanoparticles (NPs) which consists of Au NPs capped with Pt NPs (Au@Pt NPs) is constructed and functionalised with a quinazoline based small molecule (Au@Pt@Q NPs), acting as a theranostic agent against glioblastoma. Two different hydrothermal synthetic procedures for bimetallic Au@Pt NPs are presented and the resulting nanostructures are fully characterised by means of spectroscopic and microscopic methods. The imaging and targeting capacity of the new drug delivery system is assessed through fluorescent optical microscopy and cytotoxicity evaluations. The constructed Au@Pt NPs consist a monodispersed colloidal solution of 25 nm with photoluminescent, fluorescent and X-Ray absorption properties that confirm their diagnostic potential. Haemolysis testing demonstrated that Au@Pt NPs are biocompatible and fluorescent microscopy confirmed their entering the cells. Cytological evaluation of the NPs through MTT assay showed that they do not inhibit the proliferation of control cell line HEK293, whereas they are toxic in U87MG, U251 and D54 glioblastoma cell lines; rendering them selective targeting agents for treating glioblastoma.

Keywords: Glioblastoma; Gold; Nanoparticles; Platinum; Quinazoline.

MeSH terms

Drug Delivery Systems
Glioblastoma* / drug therapy
Gold / chemistry
HEK293 Cells
Humans
Metal Nanoparticles* / chemistry
Platinum / chemistry

Substances

Platinum
Gold