Shifts of lipid metabolites help decode immobilization of soil cadmium under reductive soil disinfestation

Abstract

Cadmium (Cd) contamination in soil can cause serious environmental problems and threaten human health. Previous studies have shown that the reductive soil disinfestation (RSD) is regarded as an effective soil disinfection technology, which will affect the bioavailability of Cd. However, the influence of soil microorganisms and their metabolites on the morphologies of Cd during RSD treatment are still poorly understood. Here, a laboratory incubation experiment that composed of untreated soil (CK), two RSD treatments with flooded soil (FL) and added 2% bean dregs soil (BD) was conducted. After the treatment, the content of different morphologies of Cd in the soil and the molecular characteristics (the composition of the microbial community, functional enzymes and metabolites) of the soil were measured. The study found that, compared to CK treatment, the dominant phyla, such as Acidobacteria, Bacteroidetes, Firmicutes, etc., were significantly increased in BD treatment, and enzymes related to metabolism also showed noticeable enhancement. The differential accumulated metabolite (DAM) analysis revealed that the abound of lipids and lipid-like molecules involved with fatty acyls, steroids and steroid derivatives, glycerophospholipids, fatty acids and conjugates, glycerolipids, and sphingolipids were significant different among treatments. The correlation analysis showed the exchangeable fraction cadmium contents (EX-Cd) were negatively correlated with the content of glycerophospholipids and sphingolipids, and positively correlated with glycerolipids content. However, the relationship between the residual cadmium (RS-Cd) and these three metabolites was just the opposite. Compared with another two treatments, the BD treatment significantly reduced EX-Cd contents. Biological interaction network analysis indicated that the phyla Gemmatimonadetes and Proteobacteria assumed the primary responsibility for the morphological transformation of Cd through their corresponding functional enzymes. Overall, this study provided a new perspective on RSD-mediated soil Cd immobilization, and the findings should be beneficial to further applications of RSD technology on the remediation of Cd-polluted soils.

Keywords: Cd contamination; Correlation network analysis; Metabolomes; Microbiota; Reductive soil disinfestation.