Protocol for assessing auditory brainstem response in mice using a four-channel recording system

Lingling Zeng; Wen Zhou; Dan Bing; Li Xie; Xiaodi Wang; Guo Zhang

doi:10.1016/j.xpro.2022.101251

Protocol for assessing auditory brainstem response in mice using a four-channel recording system

STAR Protoc. 2022 Mar 16;3(2):101251. doi: 10.1016/j.xpro.2022.101251. eCollection 2022 Jun 17.

Authors

Lingling Zeng¹, Wen Zhou², Dan Bing¹, Li Xie¹, Xiaodi Wang¹, Guo Zhang³

Affiliations

¹ Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
² Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.
³ Key Laboratory of Environmental Health, Ministry of Education, Department of Toxicology, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Abstract

Evaluating auditory sensitivity is critical to hearing research, particularly to that focusing on hearing impairment. Auditory brainstem response recording is frequently used in mice to assess auditory sensitivity and is an approach superior to the traditional techniques. Here, we describe a protocol for recording ABR in mice using four-channel equipment. We detail the procedures of animal preparation, the setup of the ABR recording system, the click- and tone burst-evoked ABR recordings, and data analysis.

Keywords: Behavior; Health Sciences; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Auditory Threshold / physiology
Evoked Potentials, Auditory, Brain Stem* / physiology
Hearing / physiology
Hearing Loss* / diagnosis
Hearing Tests
Mice