The proteome signature of cord blood plasma with high hematopoietic stem and progenitor cell count

Anders K Nilsson; Halfdan Rydbeck; Annika Thorsell; Sofia Frändberg; Helena Barreto Henriksson; Camilla Hesse; Gunnel Hellgren; Pia Lundgren; Ann Hellström

doi:10.1016/j.scr.2022.102752

The proteome signature of cord blood plasma with high hematopoietic stem and progenitor cell count

Stem Cell Res. 2022 May:61:102752. doi: 10.1016/j.scr.2022.102752. Epub 2022 Mar 14.

Authors

Anders K Nilsson¹, Halfdan Rydbeck², Annika Thorsell³, Sofia Frändberg⁴, Helena Barreto Henriksson⁵, Camilla Hesse⁴, Gunnel Hellgren⁶, Pia Lundgren⁷, Ann Hellström⁷

Affiliations

¹ The Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. Electronic address: anders.k.nilsson@gu.se.
² The Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; The Bioinformatics Core Facility at the University of Gothenburg, Gothenburg, Sweden.
³ Proteomics Core Facility at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
⁴ Department of Laboratory Medicine, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
⁵ Department of Laboratory Medicine, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Region Västra Götaland, Sahlgrenska University Hospital, Department of Research, Development, Education and Innovation, Sahlgrenska University Hospital, Gothenburg, Sweden.
⁶ The Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
⁷ The Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

PMID: 35313264
DOI: 10.1016/j.scr.2022.102752

Abstract

Hematopoietic stem and progenitor cells (HSPC) from umbilical cord blood (UCB) are used for transplantation to treat blood disorders. Methods to estimate the HSPC count in umbilical cord blood, and thereby identify high-value blood units, are time-consuming and costly. Recent studies indicate that the UCB plasma protein composition relates to the HSPC count. We compared the plasma proteome of UCB with high vs low HSPC cell count (>115 × 10⁶ vs < 51 × 10⁶ CD34⁺ cells l^-1) by using a combination of global untargeted MS quantitative proteomics and targeted proximity extension assay (PEA) proteomics. For the MS platform, 96 proteins differed significantly between the CD34⁺ groups, and out of these, 44 proteins showed more than a two-fold difference. Seven pathways were enriched in high CD34⁺ samples, including pathways relating to platelets, coagulation, and lipid transport. For the PEA platform, 61 proteins were differentially abundant, and among these 7 proteins showed more than a two-fold difference between groups. In the PEA data, a high CD34⁺ cell count was associated with a protein hub with functions in platelet degranulation. We conclude that the HSPC count is related to the UCB plasma proteome, but that further studies are needed to discern if these findings reflect causal relationships.

Keywords: CD34; Neonates; Proteomics; Proximity extension assay; Umbilical cord.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, CD34 / metabolism
Cell Adhesion Molecules / metabolism
Cell Count
Fetal Blood* / metabolism
Hematopoietic Stem Cell Transplantation*
Hematopoietic Stem Cells / metabolism
Plasma / metabolism
Proteome / metabolism

Substances

Antigens, CD34
Cell Adhesion Molecules
Proteome