Role of IS Kpn23 in blaBKC-1 Expression and Mobilization

Willames M B S Martins; Ezequiel A Nascimento; Rodrigo Cayô; Ana C Gales

doi:10.1128/aac.00875-21

Role of IS Kpn23 in bla_BKC-1 Expression and Mobilization

Antimicrob Agents Chemother. 2022 Apr 19;66(4):e0087521. doi: 10.1128/aac.00875-21. Epub 2022 Mar 21.

Authors

Willames M B S Martins¹, Ezequiel A Nascimento¹, Rodrigo Cayô^{1

2}, Ana C Gales¹

Affiliations

¹ Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
² Laboratório de Imunologia e Bacteriologia, Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ciências Biológicas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, Brazil.

Abstract

This study aimed to verify the role of ISKpn23 in the expression and mobilization of bla_BKC-1 and aph(3')-VIi. Five constructs related to the natural bla_BKC-1 genetic background in plasmid p60136 were made and submitted for antimicrobial susceptibility testing and quantitative reverse transcription-PCR. Transposition of ISKpn23-bla_BKC-1 was investigated using transposition assays involving a 9.7-kb nonconjugative plasmid carrying bla_BKC-1 (p60136) and a transfer-proficient plasmid (pOX38-Gen). The presence of ISKpn23 had a crucial role in bla_BKC-1 expression, resulting in increased β-lactam MICs. While we detected mobilization of p60136 by the pOX38-Gen plasmid, transposition of ISKpn23-bla_BKC-1 was not observed.

Keywords: carbapenemase; insertion sequences; plasmid; transposition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins* / genetics
Conjugation, Genetic
Microbial Sensitivity Tests
Plasmids / genetics
beta-Lactamases* / genetics
beta-Lactamases* / metabolism

Substances

Bacterial Proteins
beta-Lactamases