Study on the differences of phyllosphere microorganisms between poplar hybrid offspring and their parents

Changjun Ding; Weixi Zhang; Yanbo Wang; Mi Ding; Xiaojiang Wang; Aiping Li; Dejun Liang; Xiaohua Su

doi:10.7717/peerj.12915

Study on the differences of phyllosphere microorganisms between poplar hybrid offspring and their parents

PeerJ. 2022 Mar 15:10:e12915. doi: 10.7717/peerj.12915. eCollection 2022.

Authors

Changjun Ding^{1

2}, Weixi Zhang^{1

2}, Yanbo Wang^{1

2}, Mi Ding^{1

2}, Xiaojiang Wang³, Aiping Li³, Dejun Liang⁴, Xiaohua Su^{1

2}

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
² Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
³ Inner Mongolia Academy of Forestry Sciences, Hohhot, Inner Mongolia, China.
⁴ Liaoning Provincial Poplar Institute, Gaizhou, Liaoning, China.

Abstract

The females and males of dioecious plants have evolved sex-specific characteristics in terms of their morphological and physiological properties. However, the differentiation of phyllosphere microorganism of dioecious plants between parents and hybrid offspring remain largely unexplored. Here, the phyllosphere bacterial and fungal community diversity and composition of female (Populus nigra 'DH5' (PNDH5)), male (P. simonii 'DH4' (PSDH4)), and the hybrid offspring (P. simonii × P. nigra 'DH1' (PSPNDH1), P. simonii × P. nigra 'DH2' (PSPNDH2), P. simonii × P. nigra 'DH3' (PSPNDH3)) were investigated using 16S rDNA/ITS rDNA gene-based Illumina NovaSeq 6000 sequencing. There was considerable variation of plant height, diameter at breast height, leaf area, length of petioles, leaf moisture content, and starch among different samples, and PSDH2 owned the highest plant height, diameter at breast height, and length of petioles. No distinct differences of phyllosphere bacterial community diversity were observed among PSDH4, PNDH5, PSPNDH1, PSPNDH2, and PSPNDH3; while, PSPNDH2 owned the highest fungal Pielou_e index, Shannon index, and Simpson index. Firmicutes and Ascomycota were the predominant phyllosphere bacterial and fungal community at the phylum level, respectively. Bacilli and Gammaproteobacteria were the two most dominant bacterial classes regardless of parent and the hybrid offspring. The predominant phyllosphere fungal community was Dothideomycetes at the class level. The NMDS demonstrated that phyllosphere microbial community obviously differed between parents and offspring, while the phyllosphere microbial community presented some similarities under different hybrid progeny. Also, leaf characteristics contributed to the differentiation of phyllosphere bacterial and fungal communities between parents and hybrid offspring. These results highlighted the discrimination of phyllosphere microorganisms on parent and hybrid offspring, which provided clues to potential host-related species in the phyllosphere environment.

Keywords: Hybrid; P. nigra ‘DH5’; Parent; Phyllosphere microganism; Populus simonii ‘DH4’.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Ribosomal
Microbiota*
Mycobiome*
Plants / genetics
Populus* / genetics

Substances

DNA, Ribosomal

Grants and funding

This work was supported by the Basic Research Fund of RIF (Grant Nos. CAFYBB2020SZ002 and CAFYBB2017ZA001-3), and The National Natural Science Foundation of China (Grant No. 31870662). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.