Chinese Medicine Formula Siwu-Yin Inhibits Esophageal Precancerous Lesions by Improving Intestinal Flora and Macrophage Polarization

Hui-Juan Shi; Xuan-Yu Chen; Xin-Ran Chen; Zhong-Bing Wu; Jian-Yong Li; Ya-Qin Sun; Dong-Xuan Shi; Jing Li

doi:10.3389/fphar.2022.812386

Chinese Medicine Formula Siwu-Yin Inhibits Esophageal Precancerous Lesions by Improving Intestinal Flora and Macrophage Polarization

Front Pharmacol. 2022 Mar 3:13:812386. doi: 10.3389/fphar.2022.812386. eCollection 2022.

Authors

Hui-Juan Shi¹, Xuan-Yu Chen², Xin-Ran Chen³, Zhong-Bing Wu⁴, Jian-Yong Li⁵, Ya-Qin Sun⁶, Dong-Xuan Shi¹, Jing Li^{1

4}

Affiliations

¹ Department of Traditional Chinese Medicine, The Fourth Hospital of Hebei Medical University, Hebei Cancer Hospital, Shijiazhuang, China.
² Institute for Biotechnology, St. John's University, Queens, NY, United States.
³ Pharmacy Department, The Fourth Hospital of Hebei Medical University, Hebei Cancer Hospital, Shijiazhuang, China.
⁴ College of Integrated Traditional Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, China.
⁵ Hebei Ping An Health Group Pharmaceutical Research Institute, Shijiazhuang, China.
⁶ Health Food Research Office, Shijiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang, China.

Abstract

Siwu-Yin (SWY), a traditional Chinese medicinal formula, can replenish blood and nourish Yin. It was recorded in ancient Chinese medicine books in treating esophageal dysphagia, which has similar symptoms and prognosis with esophageal precancerous lesions and esophageal cancer. However, its effect has not been established in vivo. This study explores the antiesophageal cancer effect of SWY on rats with esophageal precancerous lesions. By performing 16S rRNA gene sequencing and metabolomics, it was suggested that SWY may improve the composition of intestinal flora of rats by regulating the synthesis and secretion of bile acids. In addition, flow cytometry results showed that SWY treatment modified tumor microenvironment by improving macrophage polarization and therefore inhibiting the occurrence of esophageal precancerous lesions.

Keywords: Siwu-Yin; esophageal precancerous lesions; macrophage polarization; metabolomics; synthesis and secretion of bile acids; traditional Chinese medicine.