Emergence of a Neisseria flavescens clinical strain with a high level of third-generation cephalosporins resistance in Lebanon

May Khoder; Rayane Rafei; Marwan Osman; Issmat I Kassem; Ahmad Shahin; Monzer Hamze; Jean-Marc Rolain

doi:10.1016/j.diagmicrobio.2022.115660

Emergence of a Neisseria flavescens clinical strain with a high level of third-generation cephalosporins resistance in Lebanon

Diagn Microbiol Infect Dis. 2022 May;103(1):115660. doi: 10.1016/j.diagmicrobio.2022.115660. Epub 2022 Feb 23.

Authors

May Khoder¹, Rayane Rafei², Marwan Osman³, Issmat I Kassem⁴, Ahmad Shahin⁵, Monzer Hamze⁶, Jean-Marc Rolain⁷

Affiliations

¹ Laboratoire Microbiologie Santé et Environnement, Doctoral School of Sciences and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon; Aix-Marseille University, IRD, APHM, MEPI, IHU Méditerranée infection, Faculté de Médecine et de Pharmacie, Marseille, France.
² Laboratoire Microbiologie Santé et Environnement, Doctoral School of Sciences and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon.
³ Laboratoire Microbiologie Santé et Environnement, Doctoral School of Sciences and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon; Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. Electronic address: mo368@cornell.edu.
⁴ Food Science and Technology, Center for Food Safety, University of Georgia, Griffin, GA, USA.
⁵ Laboratoire Microbiologie Santé et Environnement, Doctoral School of Sciences and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon; Laboratory of Informatics and their Applications, Doctoral School of Sciences and Technology, Lebanese University, Tripoli, Lebanon.
⁶ Laboratoire Microbiologie Santé et Environnement, Doctoral School of Sciences and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon. Electronic address: mhamze@monzerhamze.com.
⁷ Aix-Marseille University, IRD, APHM, MEPI, IHU Méditerranée infection, Faculté de Médecine et de Pharmacie, Marseille, France.

PMID: 35306445
DOI: 10.1016/j.diagmicrobio.2022.115660

Abstract

Third generation cephalosporins (3GC)-resistant Neisseria flavescens could cause difficult-to-treat infections. This study aimed to investigate the genetic determinants of 3GC resistance using whole genome sequence comparisons of three N. flavescens; one of which was 3GC-resistant, while two were susceptible. Mutations in penA, ponA, and porB might be associated with the 3GC-resistance.

Keywords: Antimicrobial resistance; Neisseria flavescens; Third-generation cephalosporins; Whole genome sequencing.

Publication types

Case Reports

MeSH terms

Anti-Bacterial Agents / pharmacology
Cephalosporins / pharmacology
Gonorrhea*
Humans
Lebanon
Microbial Sensitivity Tests
Neisseria
Neisseria gonorrhoeae* / genetics

Substances

Anti-Bacterial Agents
Cephalosporins

Supplementary concepts

Neisseria flavescens