Microbial fuel cells (MFCs) can obtain electrical energy from extensive organic matter and complete wastewater treatment at the same time. The principal purpose of the research is to find a solution to the biodegradation of X-3B in a double tube MFC with graphite fiber brush as the anode and carbon cloth as the cathode. The anaerobic, aerobic, and electrochemical processes in the MFC were investigated. The effects of dye concentration and circuit connectivity on the performances of MFCs were explored. The degradation efficiency of X-3B in the anode region (85.56%) was higher than that in the cathode region (14.16%) within 24 h under the optimal voltage of 0.43 V, indicating a synergistic effect between electrode reaction and biodegradation. The power density increased from 12.12 mW/m3 to 60.45 mW/m3 with the addition of X-3B from 50 to 200 mg/L, because of the reduced ohmic and polarization resistance. Intermediate productions such as aniline were manufactured with the conjugated double bond of X-3B broken, and the intermediates were degraded into small molecular products like phenol during further degradation processes. Moreover, dye concentration and circuit connection had significant effects on the relative abundance of the microbial community at phylum and genus levels. In general, MFC is a good approach to energy generation and azo dye treatment at the same time.
Keywords: Microbial fuel cell, Azo dye, Bioelectrochemical treatment, Degradation pathway, Microbial communities.
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.