Joint effects of bacterium and biochar in remediation of antibiotic-heavy metal contaminated soil and responses of resistance gene and microbial community

Xiaorong Zhang; Zongqiang Gong; Graeme Allinson; Xiaojun Li; Chunyun Jia

doi:10.1016/j.chemosphere.2022.134333

Joint effects of bacterium and biochar in remediation of antibiotic-heavy metal contaminated soil and responses of resistance gene and microbial community

Chemosphere. 2022 Jul:299:134333. doi: 10.1016/j.chemosphere.2022.134333. Epub 2022 Mar 15.

Authors

Xiaorong Zhang¹, Zongqiang Gong², Graeme Allinson³, Xiaojun Li⁴, Chunyun Jia⁵

Affiliations

¹ Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address: zhangxiaorong191@mails.ucas.ac.cn.
² Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China. Electronic address: zgong@iae.ac.cn.
³ School of Science, RMIT University, Melbourne, Victoria, 3000, Australia. Electronic address: graeme.allinson@rmit.edu.au.
⁴ Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China. Electronic address: lixiaojun@iae.ac.cn.
⁵ Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China. Electronic address: jiachunyun@iae.ac.cn.

PMID: 35304205
DOI: 10.1016/j.chemosphere.2022.134333

Abstract

Soils containing both veterinary antibiotics (VAs) and heavy metals necessitate effective remediation approaches, and microbial and molecular levels of the results should be further examined. Here, a novel material combining waste fungus chaff-based biochar (WFCB) and Herbaspirillum huttiense (HHS1) was established to immobilize copper (Cu) and zinc (Zn) and degrade oxytetracycline (OTC) and enrofloxacin (ENR). Results showed that the combined material exhibited high immobilization of Cu (85.5%) and Zn (64.4%) and great removals of OTC (41.9%) and ENR (40.7%). Resistance genes including tet(PB), tetH, tetR, tetS, tetT, tetM, aacA/aphD, aacC, aadA9, and czcA were reduced. Abundances of potential hosts of antibiotic resistance genes (ARGs) including phylum Proteobacteria and genera Brevundimonas and Rhodanobacter were altered. Total phosphorus and pH were the factors driving the VA degrading microorganisms and potential hosts of ARGs. The combination of WFCB and HHS1 can serve as an important bioresource for immobilizing heavy metals and removing VAs in the contaminated soil.

Keywords: Bacterium; Biochar; Network analysis; Resistance genes.

MeSH terms

Anti-Bacterial Agents / pharmacology
Charcoal / chemistry
Charcoal / pharmacology
Enrofloxacin
Metals, Heavy* / analysis
Metals, Heavy* / toxicity
Microbiota*
Oxytetracycline*
Soil / chemistry
Soil Pollutants* / analysis

Substances

Anti-Bacterial Agents
Metals, Heavy
Soil
Soil Pollutants
biochar
Charcoal
Enrofloxacin
Oxytetracycline