A microfluidic genoserotyping strategy for fast and objective identification of common Salmonella serotypes isolated from retail food samples in China

Abstract

Strict monitoring of Salmonella serotypes is the most effective way to limit the risk of its transmission to humans. In this study, a microfluidic genoserotyping strategy was developed for the rapid and cost-effective detection of 11 common Salmonella serotypes from retail food samples, i.e. Typhimurium, Derby, Indiana, Agona, Rissen, Braenderup, Hadar, Enteritidis, Weltevreden, Meleagridis, and Pomona. The programmable LAMP in the strategy can complete the whole detection process within 40 min and avoid false positive results. The limit of detection was 10² or 10³ CFU/mL. Referring to the results of standard culture method, the LAMP-chip was verified with 100% accuracy on testing 688 Salmonella and 22 non-Salmonella strains. This simple, portable and accurate microfluidic genoserotyping strategy is fully adapted to the needs of food regulatory authorities and has wide application prospect in food safety field.

Keywords: Food safety; Genoserotyping; Loop-mediated isothermal amplification; Salmonella.