Bioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity

Nilesh Rai; Priyanka Kumari Keshri; Priyamvada Gupta; Ashish Verma; Swapnil C Kamble; Santosh Kumar Singh; Vibhav Gautam

doi:10.1371/journal.pone.0264673

Bioprospecting of fungal endophytes from Oroxylum indicum (L.) Kurz with antioxidant and cytotoxic activity

PLoS One. 2022 Mar 17;17(3):e0264673. doi: 10.1371/journal.pone.0264673. eCollection 2022.

Authors

Nilesh Rai¹, Priyanka Kumari Keshri¹, Priyamvada Gupta¹, Ashish Verma¹, Swapnil C Kamble², Santosh Kumar Singh¹, Vibhav Gautam¹

Affiliations

¹ Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
² Department of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune, India.

Abstract

Oroxylum indicum (L.) Kurz, a medicinal plant, shows numerous pharmacological properties which may be attributed to the bioactive compounds produced by O. indicum or due to associated endophytes. In the present study, leaf of O. indicum was evaluated for the presence of associated fungal endophytes, and antioxidant and cytotoxic activities of bioactive compounds produced from them. Using culture-dependent approach, eight fungal endophytes belonging to five different genera were identified. Two endophytes Daldinia eschscholtzii and Ectophoma multirostrata have been reported for the first time from the leaf of O. indicum plant. High-performance thin-layer chromatography (HPTLC) of ethyl acetate (EA) extract of isolated fungal endophytes showed a distinct fingerprinting profile in EA extract of Colletotrichum gloeosporioides. Among identified endophytes, EA extract of C. gloeosporioides showed significant antioxidant activity against DPPH free radical, superoxide anion radical, nitric oxide radical and hydroxyl radical with EC50 values of 22.24±1.302 μg/mL, 67.46±0.576 μg/mL, 80.10±0.706 μg/mL and 61.55±1.360 μg/mL, respectively. EA extract of C. gloeosporioides exhibited potential cytotoxicity against HCT116, HeLa and HepG2 cancer cell lines with IC50 values of 76.59 μg/mL, 176.20 μg/mL and 1750.70 μg/mL, respectively. A comparative HPTLC fingerprinting and the antioxidant activity of C. gloeosporioides associated with two different hosts (leaf of O. indicum and dead twigs of other plant) showed that C. gloeosporioides produces bioactive compounds in a host-dependent manner.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / metabolism
Bignoniaceae*
Bioprospecting
Endophytes / metabolism
Fungi, Unclassified*
Plant Extracts / chemistry

Substances

Antioxidants
Plant Extracts

Grants and funding

This work is supported by the Science and Engineering Research Board (https://www.serbonline.in/) through the Empowerment and Equity Opportunities for Excellence in Science grant awarded to VG (EEQ/2019/000025). The Institution of Eminence SEED Grant, Banaras Hindu University, Varanasi, India and University Grants Commission, New Delhi, India also provided funding support to VG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.