Mobile-device-based two-dimensional measurement for estimating the embryo and endosperm areas of brown rice

Appl Opt. 2022 Mar 10;61(8):E14-E20. doi: 10.1364/AO.444854.

Abstract

The rice kernel inside a hull is composed of the embryo and endosperm. The embryo or germ of the rice seed will grow and become the shoot and the root parts of a seedling, while the endosperm is an important nutrient source for the embryo in the early stages. Hence, the health of seedlings depends particularly on the sizes of the embryo and endosperm. In this work, we propose and experimentally demonstrate how the embryo and endosperm areas of brown rice can simply be determined. Our key idea is based on the utilization of a smart mobile device equipped with our specifically designed lens module arranged in a simple cross-polarization imaging configuration for acquiring a rice grain image upon the illumination of a white light source and then spatially analyzing the sizes of embryo and endosperm areas. The prototype shows promising results in identifying the sizes of the embryo and endosperm within 2 s per seed with a measurement error of <9% compared with the use of off-the-shelf image editing software. In addition, the prototype is in a small package of 20×32.5×6.5cm3 with 4 kg weight, thus showing high potential to perform in the real scenario.

MeSH terms

  • Computers, Handheld
  • Embryo, Mammalian
  • Endosperm*
  • Oryza*
  • Seeds