The effect of stromal vascular fraction and Platelet-Rich Plasma combination on basic Fibroblast Growth Factor serum level during anal trauma healing in a Wistar rat model

Ricko Sadli Sujana; Sulmiati; Nita Mariana; Fonny Josh; Sachraswaty Rachman Laidding; Andi Alfian Zainuddin; Muhammad Faruk

doi:10.1016/j.amsu.2022.103375

The effect of stromal vascular fraction and Platelet-Rich Plasma combination on basic Fibroblast Growth Factor serum level during anal trauma healing in a Wistar rat model

Ann Med Surg (Lond). 2022 Feb 11:76:103375. doi: 10.1016/j.amsu.2022.103375. eCollection 2022 Apr.

Authors

Ricko Sadli Sujana¹, Sulmiati^{2

3}, Nita Mariana^{2

3}, Fonny Josh^{4

5}, Sachraswaty Rachman Laidding^{4

5}, Andi Alfian Zainuddin⁶, Muhammad Faruk¹

Affiliations

¹ Department of Surgery, Hasanuddin University, Makassar, Indonesia.
² Division of Pediatric Surgery, Department of Surgery, Hasanuddin University, Makassar, Indonesia.
³ Division of Pediatric Surgery, Department of Surgery, Wahidin Sudirohusodo Hospital, Makassar, Indonesia.
⁴ Division of Plastic and Reconstructive Surgery, Department of Surgery, Hasanuddin University, Makassar, Indonesia.
⁵ Division of Plastic and Reconstructive Surgery, Department of Surgery, Wahidin Sudirohusodo Hospital, Makassar, Indonesia.
⁶ Department of Public Health and Community Medicine Science, Hasanuddin University, Makassar, Indonesia.

Abstract

Introduction: Stromal Vascular Fraction (SVF) and Platelet-Rich Plasma (PRP) application play important roles in the healing process by increasing basic Fibroblast Growth Factor (bFGF) secretion. This research assesses the effect of combined SVF and PRP local injection on bFGF levels, using an anal trauma model in Wistar rats.

Method: Twenty-eight adult Wistar rats were divided into three groups. Groups A and B underwent modified surgical anal trauma and repair; Group A was treated with the SVF and PRP combination local injection, while Group B was treated with only normal saline. Subsequently, we examined bFGF levels in Groups A and B on days 1, 7, and 14. Group C consisted of healthy controls sacrificed on day 0 to obtain baseline data on bFGF levels.

Results: The bFGF levels were higher in Group A than in Group B on every experimental day. The Repeated Measures test shows a significant increase in bFGF levels on day 1 (p = 0.000), day 7 (p = 0.000), and day 14 (p = 0.000). This test also indicates that the local injection combination of SVF and PRP increased bFGF levels by 96.2% compared to the placebo group.

Conclusion: The combination of SVF and PRP can increase bFGF levels during anal trauma healing in the Wistar rat model. Basic FGF is an important factor throughout the anal trauma healing process.

Keywords: Anal trauma; Basic fibroblast growth factor; Platelet-rich plasma; Stromal vascular fraction; Wound healing.