ZIKV B-cell epitopes for immunodiagnostic tests

J Immunol Methods. 2022 May:504:113246. doi: 10.1016/j.jim.2022.113246. Epub 2022 Mar 11.

Abstract

The use of serological tests is valuable to diagnose Zika virus (ZIKV) infection and carry out epidemiological surveillance. However, ZIKV serological tests may result in false positives due to cross-reactivity between antibodies against other Flavivirus, especially dengue virus that worldwide disseminated. We used three online tools to predict amino acid sequences of B-cell epitopes. We selected and synthetized two epitopes that showed appropriate features in the molecular dynamic simulation and demonstrated to be suitable for serological assays.

Keywords: Arbovirus; Bioinformatics; Immunoassay; Peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral
  • Cross Reactions
  • Dengue Virus*
  • Dengue*
  • Epitopes, B-Lymphocyte
  • Humans
  • Serologic Tests
  • Zika Virus Infection*
  • Zika Virus*

Substances

  • Antibodies, Viral
  • Epitopes, B-Lymphocyte