Disease resistance and growth promotion activities of chitin/cellulose nanofiber from spent mushroom substrate to plant

Hujun Li; Saori Yoshida; Naofumi Mitani; Mayumi Egusa; Momoko Takagi; Hironori Izawa; Teruyuki Matsumoto; Hironori Kaminaka; Shinsuke Ifuku

doi:10.1016/j.carbpol.2022.119233

Disease resistance and growth promotion activities of chitin/cellulose nanofiber from spent mushroom substrate to plant

Carbohydr Polym. 2022 May 15:284:119233. doi: 10.1016/j.carbpol.2022.119233. Epub 2022 Feb 8.

Authors

Hujun Li¹, Saori Yoshida¹, Naofumi Mitani¹, Mayumi Egusa², Momoko Takagi², Hironori Izawa³, Teruyuki Matsumoto⁴, Hironori Kaminaka², Shinsuke Ifuku⁵

Affiliations

¹ Department of Engineering, Graduate School of Sustainability Science, Tottori University, 4-101 Koyama-Minami, Tottori 680-8552, Japan.
² Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.
³ Department of Engineering, Graduate School of Sustainability Science, Tottori University, 4-101 Koyama-Minami, Tottori 680-8552, Japan; Center for Research on Green Sustainable Chemistry, Tottori University, 4-101 11 Koyama-Minami, Tottori 680-8550, Japan.
⁴ Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan; Marine Nano-fiber Co., Ltd., 4-101 Koyama-Minami, Tottori 680-8550, Japan.
⁵ Department of Engineering, Graduate School of Sustainability Science, Tottori University, 4-101 Koyama-Minami, Tottori 680-8552, Japan; Center for Research on Green Sustainable Chemistry, Tottori University, 4-101 11 Koyama-Minami, Tottori 680-8550, Japan. Electronic address: sifuku@tottori-u.ac.jp.

PMID: 35287923
DOI: 10.1016/j.carbpol.2022.119233

Abstract

Some studies have reported the method for treating the spent mushroom substrate (SMS). However, the effective use as a functional raw material based on properties of SMS remains a formidable challenge. In this study, we investigated the usefulness of SMS in agriculture to develop a new method for treating and utilizing it. First, we attempted to isolate chitin/cellulose nanofiber complex (CCNFC) from SMS using chemical pretreatment and mechanical fibrillation. The characterization results like SEM, FT-IR, and XRD showed that we successfully isolated the CCNFC from SMS. Second, we explored the biological activities of the CCNFC for its potential application as a functional agricultural nanomaterial. CCNFC water dispersion with low concentration (0.1 and 1 mg/mL) exhibited significant plant disease resistance and plant growth promotion activities. Our results suggested that SMS may provide a useful source of functional agricultural nanomaterial, which may contribute to treating and applying it in agriculture.

Keywords: Cellulose nanofibers; Chitin nanofibers; Functional agricultural nanomaterial; Plant disease resistance; Plant growth promotion; Spent mushroom substrate.

MeSH terms

Agaricales* / chemistry
Cellulose
Chitin
Disease Resistance
Nanofibers*
Spectroscopy, Fourier Transform Infrared

Substances

Chitin
Cellulose