The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility

Sihan Zhang; Guanglei Zhao; Wei Ma; Yanghui Song; Cheng Huang; Chong Xie; Kebing Chen; Xiaofeng Li

doi:10.1016/j.carbpol.2022.119255

The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility

Carbohydr Polym. 2022 Jun 1:285:119255. doi: 10.1016/j.carbpol.2022.119255. Epub 2022 Feb 17.

Authors

Sihan Zhang¹, Guanglei Zhao², Wei Ma¹, Yanghui Song¹, Cheng Huang¹, Chong Xie¹, Kebing Chen³, Xiaofeng Li⁴

Affiliations

¹ State Key Lab of Pulp and Papermaking Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China.
² State Key Lab of Pulp and Papermaking Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China. Electronic address: glzhao@scut.edu.cn.
³ Department of Spine Surgery, Center for Orthopaedic Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, China. Electronic address: chkbing@mail.sysu.edu.cn.
⁴ School of Food Science and Engineering, South China University of Technology, Guangzhou 510644, China. Electronic address: xflibio@scut.edu.cn.

PMID: 35287869
DOI: 10.1016/j.carbpol.2022.119255

Abstract

Bone tissue repair is difficult due to the dense structure of the extracellular matrix. To solve this problem, a porous chitosan nanofiber scaffold (CSNFS) with an extracellular matrix-like structure was prepared via a facile cross-linked reaction of root-like chitosan nanofiber (CSNF) and collagen (Col) by using genipin (Gen) as the cross-linker. The optimal preparation conditions of CSNFS is weight ratio of CSNF:Col:Gen =1:1:0.1, crosslinked 48 h under 37 °C. CSNFS shows high porosity with adequate micro-scale pores, and its BET data shows that there are a large number of nano-scale pores. The CSNFS mechanical strength is higher than that of the chitosan scaffold both in dry and wet state. MC3T3 cells grow well on CSNFS, can overgrow the scaffold in three-dimensional space, adhere and differentiate well within those nanofiber structure. The cross-linked CSNFS has good biocompatibility and can be used as a repair material for bone tissue engineering.

Keywords: Bone tissue engineering; Chitosan nanofiber; Collagen; Genipin; Scaffold.

MeSH terms

Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology
Chitosan* / chemistry
Chitosan* / pharmacology
Collagen Type I / chemistry
Iridoids
Nanofibers* / chemistry
Osteoblasts
Porosity
Tissue Engineering / methods
Tissue Scaffolds / chemistry

Substances

Biocompatible Materials
Collagen Type I
Iridoids
Chitosan
genipin