Colorimetric detection of thrombin based on signal amplification by transcription-reverse transcription concerted reaction using non-crosslinking aggregation of gold nanoparticles

Yu Muto; Tamotsu Zako

doi:10.1007/s44211-022-00050-5

Colorimetric detection of thrombin based on signal amplification by transcription-reverse transcription concerted reaction using non-crosslinking aggregation of gold nanoparticles

Anal Sci. 2022 Jan;38(1):3-7. doi: 10.1007/s44211-022-00050-5. Epub 2022 Feb 17.

Authors

Yu Muto^{1

2}, Tamotsu Zako³

Affiliations

¹ Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, 790-8577, Japan.
² Tokyo Research Center, TOSOH Corporation, 2743-1 Hayakawa, Ayase, 252-1123, Japan.
³ Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, 790-8577, Japan. zako.tamotsu.us@ehime-u.ac.jp.

PMID: 35287200
DOI: 10.1007/s44211-022-00050-5

Abstract

Gold nanoparticles (AuNPs) have been used as colorimetric biosensors by utilizing the difference in color between the dispersed (red) and aggregated (blue) states. We previously developed a biosensor that converts sandwich-type thrombin recognition to RNA amplification and color difference of AuNPs. But the sensitivity was insufficient because of the linear signal amplification mechanism. In this study, we designed an exponential signal amplification biosensor based on transcription-reverse transcription concerted (TRC) reaction.

Keywords: Aptamer; Gold nanoparticle; Signal amplification; Thrombin; Transcription-reverse transcription concerted (TRC) reaction.

MeSH terms

Biosensing Techniques*
Colorimetry / methods*
Gold
Metal Nanoparticles*
Reverse Transcription
Thrombin / analysis*

Substances

Gold
Thrombin