HiSAT: A Novel Method for the Rapid Diagnosis of Allergy

Hirotomo Shibaguchi; Yuki Yasutaka

doi:10.21769/BioProtoc.4309

HiSAT: A Novel Method for the Rapid Diagnosis of Allergy

Bio Protoc. 2022 Feb 5;12(3):e4309. doi: 10.21769/BioProtoc.4309.

Authors

Hirotomo Shibaguchi^{1

2}, Yuki Yasutaka^{2

3}

Affiliations

¹ Department of Biochemistry, Faculty of Medicine, Fukuoka University., Fukuoka, Japan.
² Department of Pharmacy, Fukuoka University Hospital, Fukuoka, Japan.
³ Department of Health Care Management, Faculty of Pharmaceutical Science, Fukuoka University, Fukuoka, Japan.

Abstract

To identify causative substances for allergies to drugs or foods, the lymphocyte transformation test (LTT) is currently widely used as in vitro test, but its accuracy is not satisfactory. We have developed a novel method designated high-sensitivity allergy test (HiSAT) for determining allergy expression by measuring cell kinetics, using the chemotactic cells from non-allergic volunteers against a gradient field of cytokines released from immune cells when allergy develops. HiSAT requires a very small sample of 5 µL or less, and is applicable to three types of tests, depending on the situation in clinical practice: (i) diagnosis of the allergic expression, (ii) identification of the causative drug, and, in principle, (iii) pre-inspection.

Keywords: Allergy; Causative drug; Cell kinetics; Diagnosis; Humoral factor; In vitro test; Pre-inspection.