hsa_circ_0072309 Expression Profiling in Non-small-Cell Lung Carcinoma and Its Implications for Diagnosis and Prognosis

Yong Zhou; Zhongkai Tong; Xiaoxiao Zhu; Shaoqing Huang; Zhaoxing Dong; Zhenyue Ye

doi:10.3389/fsurg.2022.842292

hsa_circ_0072309 Expression Profiling in Non-small-Cell Lung Carcinoma and Its Implications for Diagnosis and Prognosis

Front Surg. 2022 Feb 25:9:842292. doi: 10.3389/fsurg.2022.842292. eCollection 2022.

Authors

Yong Zhou^{1

2}, Zhongkai Tong^{1

2}, Xiaoxiao Zhu^{1

2}, Shaoqing Huang^{1

2}, Zhaoxing Dong^{1

2}, Zhenyue Ye^{1

2}

Affiliations

¹ Respiratory Department, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China.
² Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo, China.

Abstract

Circular RNAs (circRNAs), which fall into the category of endogenous ncRNAs, are linked to disease progression of neoplastic diseases. Whereas, it remains uncharacterized regarding hsa_circ_0072309's function and implications in lung carcinoma (LC). Gene Expression Omnibus (GEO) database was utilized for identifying circRNAs with aberrantly expression in LC. qRT-PCR was responsible for determining hsa_circ_0072309 levels in lung adenocarcinoma (LAC). Also, its involvement in LC cell progression was investigated. Experimentally, hsa_circ_0072309 was identified as one of the most aberrantly down-regulated circRNAs in the GEO database (GSE101684 and GSE112214). qRT-PCR revealed notably down-regulated hsa_circ_0072309 in LAC tissue, which had a close association with adverse 3-year survival, as well as LNM and advanced TNM stage. Based on ROC, the AUC of hsa_circ_0072309 was determined to be 0.887, and its specificity and susceptibility can be improved by combined detection of either CYFRA21-1 or CEA. In a word, hsa_circ_0072309 is lowly expressed in lung cancer patients and the survival rate of lowly expressed patients is significantly lower, a candidate marker with prognostic utility for the disease.

Keywords: GEO; NSCLC; diagnosis; hsa_circ_0072309; prognosis.