Research on Hotair and 7SL-RNA Gene Expression Levels in Psoriasis Vulgaris

Seyma Yazıcı; Rukiye Yasak Guner; Melih Akyol; Elif Burcu Tuzemen Bayyurt; Serdal Arslan

doi:10.4103/ijd.ijd_1125_20

Research on Hotair and 7SL-RNA Gene Expression Levels in Psoriasis Vulgaris

Indian J Dermatol. 2021 Nov-Dec;66(6):704. doi: 10.4103/ijd.ijd_1125_20.

Authors

Seyma Yazıcı¹, Rukiye Yasak Guner¹, Melih Akyol¹, Elif Burcu Tuzemen Bayyurt², Serdal Arslan²

Affiliations

¹ Department of Dermatology, Cumhuriyet University School of Medicine, Sivas, Turkey.
² Department of Medical Biology, Cumhuriyet University School of Medicine, Sivas, Turkey.

Abstract

Backgrounds: Mutation of protein-coding genes and non-coding genes is a factor in psoriasis etiology. Non-coding RNA (ncRNA), which does not have protein-coding capacity, is available in the human genome. HOTAIR (HOX Antisense Intergenic RNA) and 7SL-RNA are known as ncRNA. They may play a role in psoriasis pathogenesis.

Aims: In our study, we aimed to investigate the level of HOTAIR and 7SL-RNA gene expression in the lesional and perilesional healthy skin of psoriasis patients.

Methods: Total RNA isolation from the skin samples was achieved by modifying the RNeasy Mini Kit (Qiagen, Cat No: 74104) protocol. Real Time Polymerase Chain Reaction (qPCR) phase was performed in accordance with the protocol of the relevant brand (WizPure qPCR).

Results: 7SL-RNA gene expression decreased in the skin with psoriatic lesions (FC: 0.01; p: 0.028), and this decrease was statistically significant. HOTAIR gene expression decreased (FC: 0.92; p: 0.218), but this decrease was not statistically significant.

Conclusions: lncRNAs may play a role in the pathogenesis of psoriasis disease.

Keywords: 7SL-RNA; HOTAIR; non-coding genes; psoriasis.