Four new withanolides with anti-inflammatory activity from Datura inoxia Mill. leaves

Steroids. 2022 Jun:182:109010. doi: 10.1016/j.steroids.2022.109010. Epub 2022 Mar 11.

Abstract

Four previously undescribed withanolides, datinolides A-D (1-4) and eight known withanolides (5-12), were separated from the 70% ethyl alcohol extract of Datura inoxia Mill. leaves. All structures were clarified by comprehensive spectroscopic analysis. Furthermore, all withanolides were assessed for their anti-inflammatory activity and results showed that 1 exhibited a fairly good suppression against nitric oxide generation in lipopolysaccharide-stimulated RAW 264.7 cells (IC50 = 10.33 ± 1.53 μM).

Keywords: Anti-inflammatory activities; Datura inoxia; Solanaceae; Withanolide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology
  • Datura metel* / chemistry
  • Datura* / chemistry
  • Mice
  • Plant Leaves / chemistry
  • RAW 264.7 Cells
  • Withanolides* / chemistry
  • Withanolides* / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Withanolides