Zeolite mediated processing of nitrogenous waste in the rearing environment influences gut and sediment microbial community in freshwater crayfish (Cherax cainii) culture

Md Javed Foysal; Thi Thu Thuy Nguyen; Mavis Sialumano; Simon Phiri; Md Reaz Chaklader; Ravi Fotedar; Marthe Monique Gagnon; Alfred Tay

doi:10.1016/j.chemosphere.2022.134276

Zeolite mediated processing of nitrogenous waste in the rearing environment influences gut and sediment microbial community in freshwater crayfish (Cherax cainii) culture

Chemosphere. 2022 Jul:298:134276. doi: 10.1016/j.chemosphere.2022.134276. Epub 2022 Mar 9.

Authors

Md Javed Foysal¹, Thi Thu Thuy Nguyen², Mavis Sialumano³, Simon Phiri³, Md Reaz Chaklader⁴, Ravi Fotedar⁵, Marthe Monique Gagnon⁵, Alfred Tay⁶

Affiliations

¹ School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. Electronic address: mjfoysal-geb@sust.edu.
² School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Department of Experimental Biology, Research Institute for Aquaculture No. 2, Ho Chi Minh City, Viet Nam. Electronic address: thuthuyria2@gmail.com.
³ School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Department of Veterinary Services, Ministry of Fisheries and Livestock, Zambia.
⁴ School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; Department of Primary Industries and Regional Development, Fleet Street, Fremantle, WA, Australia.
⁵ School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
⁶ Marshall Centre for Infectious Disease Research and Training, University of Western Australia, WA, Australia.

PMID: 35278449
DOI: 10.1016/j.chemosphere.2022.134276

Abstract

Zeolite is known to uptake toxic metals and filter nitrogenous waste from aquaculture effluents. The present study aimed to investigate the impacts of zeolite in three different applications namely, dietary zeolite (DZ), suspended zeolite (SZ) in the water column, and a combination of both (DZSZ) relative to unexposed freshwater crayfish, marron (control). At the end of the 56-days trial, the impact was assessed in terms of characterization of microbial communities in the culture environment and the intestine of marron. Alongside the microbial communities, the innate immune response of marron was also evaluated. The 16S rRNA data showed that marrons exposed to the suspended zeolite had a significant increase of bacterial diversity in the gut, including the restoration of marron core operational taxonomic units (OTUs), relative to other forms of exposures (DZ, DZSZ) and the control. Suspended zeolite alone also increased the number of unshared OTUs and genera, and improved predicted metabolic functions for the biosynthesis and digestion of proteins, amino acids, fatty acids, and hormones. In the tank sediment, the shift of microbial communities was connected more strongly with the time of experiment than the type of zeolite exposure. In the second case, only control marron had a different microbial ordination in terms of rare taxa present in the community. Nevertheless, the modulation in the gut environment was found more prominent in DZ, relative to modulation in the tank sediments. The taxa-environment correlation identified Rhodoferax as the most potential bacteria in removing nitrogenous waste from the rearing environment. Further analysis showed that SZ resulted in the upregulation of genes associated with the innate immune response of marron. Overall results suggest that SZ can be used to enrich microbial communities in the gut and tank sediments and better immune performance of marron.

Keywords: Aquaculture; Marron; Microbial communities; Waste removal; Zeolite.

MeSH terms

Animal Feed / analysis
Animals
Astacoidea / physiology
Fresh Water
Microbiota*
Nitrogen
RNA, Ribosomal, 16S / genetics
Zeolites*

Substances

RNA, Ribosomal, 16S
Zeolites
Nitrogen