In response to the insufficient supply of carbon sources and the toxicity of heavy metal ions when using sulfate reducing bacteria (SRB) to treat acid mine wastewater (AMD), the immobilized particles are prepared with Rhodopseudomonas, SRB and lignite as the main raw materials. And based on single factor test and orthogonal test to determine the optimal ratio of biologically activated lignite fixed SRB particles. The adsorption characteristics of immobilized particles were studied under the optimal ratio, and the reaction kinetics and adsorption capacity of SRB particles immobilized on biologically activated lignite to different ions were analyzed. The results show that: lignite not only has good adsorption performance, but also can be used as the carbon source of SRB after being degraded by Rhodopseudomonas, solving the problems of low removal efficiency of SRB treatment of AMD and insufficient carbon source supply. When the dosage of lignite (particle size is 200 mesh), Rhodopseudomonas, and SRB are 3%, 10%, and 10% mesh, the prepared biologically activated lignite-immobilized SRB particles have the best effect on AMD treatment. The removal rates of SO42-, Zn2+, and Cu2+ were 83.21%, 99.59%, and 99.93%, respectively, the pH was increased to 7.43, the COD release was 523 mg/L, and the ORP value was - 134 mV. The reduction process of SO42- by the biologically activated lignite-immobilized SRB particles conforms to the pseudo-first-order kinetics, and the adsorption of Zn2+ is more in line with the Freundlich isotherm adsorption equation and the pseudo-second-order kinetic model. And it does not spread in a single form, both internal and external diffusion occur. SEM, FT-IR, and BET analysis of biologically activated lignite immobilized SRB particles showed that the pore structure is developed, has a large number of adsorption sites, and some activated groups participate in the reaction. The adsorption process of Zn2+ and Cu2+ in AMD meets the multi-layer adsorption theory.
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