Aquaculture wastewater contained a high remnant of oxytetracycline (OTC) and nitrate. In this study, OTC co-metabolized with denitrification/desulfurization was investigated in terms of kinetic analysis, pathway, microbial communities and produces analysis in sulfate-reducing bacteria (SRB) mediated system. Long-term acclimatization with sulfate (300 mg-S/L) could markedly accelerate the removed rate of OTC from 0.9 to 1.4 mg/g-SS/d, with the kinetic constants increasing from 0.2760 to 0.5232 d-1, mainly via enzymes including adenosine-5'-phos-phosulfate reductase and cytochrome P450, and non-enzymatic process related to intermediates (adenosine-5'-phos-phosulfate and S0). Furthermore, OTC was likely detoxified by SRB enriched sludge mainly via hydrolysis, dehydration, oxidation and reduction. The denitrification process would postpone the OTC degradation via outcompeting electron donors with the desulfurization process. Redundancy analysis suggested that sulfur-oxidizing bacteria (Acidithiobacillus, Ochrobactrum) were highly related to OTC degradation processes. This study provides deep insight and a new opportunity for the treatment of aquaculture wastewater containing OTC, sulfate and nitrate by SRB sludge.
Keywords: Bio-detoxification; Co-metabolism; Combined pollution; Oxytetracycline; Sulfate reduction.
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