Anthropogenic pollution and global climate change have resulted in favorable environmental conditions for increased frequency and duration of cyanobacterial blooms in aquatic systems. Cyanobacteria can produce toxic metabolites called cyanotoxins, which have become a worldwide concern as they threaten human and animal health. The presence of cyanobacteria and four cyanotoxins were evaluated in a Colombian reservoir. The reservoir was monitored for a year, with sampling campaigns every 3 months in seven stations. To identify and quantify cyanotoxins, the ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) technique was used, and the quantification of cyanobacteria was done by quantitative real-time PCR (qPCR) assay using a cyanobacterial-specific 16S rRNA gene fragment as a target. Cyanobacteria concentration was between 4.02 (± 0.11) × 104 and 2.72 (± 0.28) × 107 copies of Cyan 16S/μL, the minimum value corresponds to the station located in the central zone and the maximum to the station at the entrance of one of the tributary rivers. The presence of MC-RR, MC-LR, MC-YR, and NOD was detected in at least six of the seven sampling stations at different times of the year. In all cases, the concentration of the toxins detected was below 0.05 μg/L, so the guideline value established by the WHO for MC-LR was not exceeded.
Keywords: Colombia; Cyanobacteria; Cyanotoxins; Tropical reservoir.
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.