Bacterial microbiota of the contact lens surface and associated care behaviours

Lunla Udomwech; Kulwadee Karnjana; Juntamanee Jewboonchu; Phisut Rattanathamma; Udomsak Narkkul; Jakkrit Juhong; Auemphon Mordmuang

doi:10.1016/j.heliyon.2022.e09038

Bacterial microbiota of the contact lens surface and associated care behaviours

Heliyon. 2022 Mar 1;8(3):e09038. doi: 10.1016/j.heliyon.2022.e09038. eCollection 2022 Mar.

Authors

Lunla Udomwech^{1

2}, Kulwadee Karnjana¹, Juntamanee Jewboonchu¹, Phisut Rattanathamma², Udomsak Narkkul¹, Jakkrit Juhong^{1

2}, Auemphon Mordmuang¹

Affiliations

¹ School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand.
² Walailak University Hospital, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Abstract

Introduction: Contact lens (CL) wear has been reported to cause changes to the microbiome of the ocular surface. More insight into the alteration of this microenvironment can help to understand the pathogenesis of CL-related eye infections. Knowledge of the relationship between the CL wearer's behaviours and pathogens would help health care providers focus on each step of proper CL care. This study aims to determine the behaviours that might be associated with the community of bacteria on CL.

Methods: A cross-sectional design was performed using anonymous questionnaires to obtain demographic data and assess hygiene practices among volunteering wearers. The CLs used were collected to evaluate the prevalence of pathogenic bacteria associated with ocular infections by PCR and microbiota analysis.

Results: The bacterial microbiota study revealed a total of 19 genera and 26 isolated strains from 20 eligible CLs. Enterobacter, Staphylococcus, and Achromobacter were the main genus in this subject population. Staphylococcus pasteuri and Achromobacter agilis were the most common pathogens at 65% and 35%, respectively. Enterobacter mori, a nonpathogenic organism, was found to be the most predominant strain, accounting for 27.51% of the total bacterial constituents. The risk behaviour of CL wear that was significantly associated with A. agilis contamination was cleaning the CL case with tap water (P value = 0.04).

Conclusions: This is the first study focusing on the association between the culture selected microbial community on the CL surface and compehensive behavioural characteristics. Environmental contamination was the main source of microbes found on CL surfaces. An emphasis in patient education should be placed on careful handling during the CL care routine and managing the hygiene of the surroundings.

Keywords: Bacterial microbiota; Contact lens; Contact lens care behaviour; Cuture-selected microbial community; Ocular infections.