Biosynthesis and Characterization of Silver Nanoparticles Produced by Phormidium ambiguum and Desertifilum tharense Cyanobacteria

Amira L Hanna; Hayam M Hamouda; Hanan A Goda; Mahmoud W Sadik; Farahat S Moghanm; Adel M Ghoneim; Muneefah A Alenezi; Sultan F Alnomasy; Pravej Alam; Tarek R Elsayed

doi:10.1155/2022/9072508

Biosynthesis and Characterization of Silver Nanoparticles Produced by Phormidium ambiguum and Desertifilum tharense Cyanobacteria

Bioinorg Chem Appl. 2022 Feb 28:2022:9072508. doi: 10.1155/2022/9072508. eCollection 2022.

Authors

Affiliations

¹ Microbiology Department, Division of Basic Medical Science, Egyptian Drug Authority EDA (National Organization for Drug Control and Research NODCAR), Giza 12553, Egypt.
² Department of Microbiology, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
³ Department of Environmental Biotechnology, College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
⁴ Soil and Water Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
⁵ Agricultural Research Center, Field Crops Research Institute, Giza 12112, Egypt.
⁶ Biology Department, Faculty of Science, Tabuk University, Tabuk 71491, Saudi Arabia.
⁷ Medical Laboratories Department, College of Applied Medical Sciences, Al- Quwayiyah, Shaqra University, Riyadh, Saudi Arabia.
⁸ Department of Biology, College of Science and Humanities, Prince Sattam Bin Abdulaziz University, Al-Kharj 1942, Saudi Arabia.

Abstract

The world faces a challenge with the pervasion of multidrug-resistant bacteria that encourages scientists to develop and discover alternative, ecofriendly, and easy-to-produce new antibacterial agents. Our work is part of the greater effort of scientists around the world to achieve this goal by the biological synthesis of silver nanoparticles using cyanobacterial extracellular and intracellular components as nonchemical reducing agents. Two Egyptian cyanobacteria were isolated and identified according to 16S rRNA gene sequencing as Phormidium ambiguum and a novel species Desertifilum tharense. The sequences were deposited with accession numbers MW762709 and MW762710 for Desertifilum tharense and Phormidium ambiguum, respectively, in the GenBank. The results of UV-Vis analysis showed promising extracellular Ag-NPs synthesis by Desertifilum tharense and Phormidium ambiguum under light conditions. Therefore, these Ag-NPs were characterized and evaluated for antibacterial and antioxidant activity. TEM and SEM analyses revealed the spherical crystals with face-centered cubic structures and size range of 6.24-11.4 nm and 6.46-12.2 nm for Ag-NPs of Desertifilum tharense and Phormidium ambiguum, respectively. XRD and EDX results confirmed the successful synthesis of Ag-NPs in their oxide form or chloride form. The FTIR spectrum data confirmed the presence of hydroxyl and amide groups. Desertifilum tharense Ag-NPs displayed the largest inhibition zone that ranged from 9 mm against Micrococcus luteus ATCC 10240 to 25 mm against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300. For Phormidium ambiguum Ag-NPs, the inhibition zone diameter was in the range of 9 mm to 18 mm. The biosynthesized Ag-NPs significantly inhibited the growth of medically important resistance-pathogenic Gram-positive and Gram-negative bacteria. The Ag-NPs of Phormidium ambiguum exhibited the highest scavenging activity of 48.7% when compared with that of Desertifilum tharense, which displayed 43.753%.