PET in vivo generators 134Ce and 140Nd on an internalizing monoclonal antibody probe

Sci Rep. 2022 Mar 9;12(1):3863. doi: 10.1038/s41598-022-07147-x.

Abstract

The in vivo-generator radionuclides 140Nd (t1/2 = 3.4 d) and 134Ce (t1/2 = 3.2 d) were used to trace a urokinase-type plasminogen activator (uPA)-targeting mouse monoclonal antibody, ATN-291, in U87 MG xenograft tumor-bearing mice. ATN-291 is known to internalize on the uPA/uPA-receptor pair, making it an appropriate targeting vector for investigating the fate of in vivo generator daughters on internalizing probes. Ante-mortem and post-mortem PET imaging at 120 h post-injection gave no indication of redistribution of the positron emitting daughter nuclides 134La and 140Pr from tumor tissue (p > 0.5). The lack of redistribution indicates that the parent radionuclides 134Ce and 140Nd could be considered as long-lived PET-diagnostic matches to therapeutic radionuclides like 177Lu, 161Tb and 225Ac when internalizing bioconjugates are employed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Humans
  • Mice
  • Neoplasms*
  • Positron-Emission Tomography / methods
  • Radioisotopes
  • Radiopharmaceuticals
  • Receptors, Urokinase Plasminogen Activator
  • Urokinase-Type Plasminogen Activator

Substances

  • Antibodies, Monoclonal
  • Radioisotopes
  • Radiopharmaceuticals
  • Receptors, Urokinase Plasminogen Activator
  • Urokinase-Type Plasminogen Activator