Protoplast Isolation and Transfection in Rice

Zhaohui Zhong; Yong Zhang

doi:10.1007/978-1-0716-2164-6_6

Protoplast Isolation and Transfection in Rice

Methods Mol Biol. 2022:2464:83-90. doi: 10.1007/978-1-0716-2164-6_6.

Authors

Zhaohui Zhong¹, Yong Zhang²

Affiliations

¹ Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
² Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China. zhangyong916@uestc.edu.cn.

PMID: 35258826
DOI: 10.1007/978-1-0716-2164-6_6

Abstract

Rice (Oryza sativa) is an important cereal crop and a model monocot plant for biology research. The reliable system of foreign DNA transformation and expression is a valuable strategy for basic research and molecular breeding application in rice. The Agrobacterium tumefaciens-mediated foreign DNA transformation system was a powerful tool for genetic research. However, it needs a long period to obtain the stable transformants for further analysis and the transformation rate limits in some organism. Protoplasts are plant cells without a cell wall, and it is much easier for foreign DNA transformation and expression. It has been widely applied in transient expression. Here, we describe a simple method for efficient protoplast isolation and transfection in rice.

Keywords: DNA transformation; Dark-grown seedling; Oryza sativa; Polyethylene glycol; Protoplast; Transient expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens / genetics
Oryza* / genetics
Plants, Genetically Modified / genetics
Protoplasts* / metabolism
Transfection
Transformation, Genetic