A two-photon fluorogenic probe based on a coumarin schiff base for formaldehyde detection in living cells

Yibin Zhang; Xianyu Qiu; Lin Sun; Qin Yan; Rudy L Luck; Haiying Liu

doi:10.1016/j.saa.2022.121074

A two-photon fluorogenic probe based on a coumarin schiff base for formaldehyde detection in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jun 5:274:121074. doi: 10.1016/j.saa.2022.121074. Epub 2022 Feb 28.

Authors

Yibin Zhang¹, Xianyu Qiu², Lin Sun², Qin Yan², Rudy L Luck³, Haiying Liu⁴

Affiliations

¹ College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, PR China. Electronic address: Yibinz@mtu.edu.
² College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, PR China.
³ Department of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: rluck@mtu.edu.
⁴ Department of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: hyliu@mtu.edu.

PMID: 35257990
DOI: 10.1016/j.saa.2022.121074

Abstract

A novel two-photon fluorogenic probe has been developed to detect formaldehyde with fast response, low cytotoxicity, and excellent selectivity. This probe exhibits a strong turn-on fluorescence response to formaldehyde under excitation at 370 nm and has been successfully applied to detect formaldehyde in living cells. Theoretical calculations at the B3LYP/6-31 + G(d,p), APFD/6-311++G, and APFD/6-311 + G(2d,p) levels of theory for the absorption and emission wavelengths of the probes were in agreement with those obtained experimentally.

Keywords: Coumarin fluorophore; Fluorogenic probe; Formaldehyde; Living cell; Two-photon excitation microscopy.

MeSH terms

Coumarins
Fluorescent Dyes*
Formaldehyde
HeLa Cells
Humans
Schiff Bases*

Substances

Coumarins
Fluorescent Dyes
Schiff Bases
Formaldehyde
coumarin