SP protects Nile tilapia (Oreochromis niloticus) against acute Streptococcus agalatiae infection

Qi Li; Baijian Jiang; Zhiqiang Zhang; Yongxiong Huang; Zhou Xu; Xinjin Chen; Yu Huang; Jichang Jian

doi:10.1016/j.fsi.2022.03.002

SP protects Nile tilapia (Oreochromis niloticus) against acute Streptococcus agalatiae infection

Fish Shellfish Immunol. 2022 Apr:123:218-228. doi: 10.1016/j.fsi.2022.03.002. Epub 2022 Mar 4.

Authors

Qi Li¹, Baijian Jiang¹, Zhiqiang Zhang¹, Yongxiong Huang¹, Zhou Xu¹, Xinjin Chen¹, Yu Huang², Jichang Jian³

Affiliations

¹ College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China.
² College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China. Electronic address: huangyu@gdou.edu.cn.
³ College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animal, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen, China.

PMID: 35257891
DOI: 10.1016/j.fsi.2022.03.002

Abstract

Substance P (SP) is a neuropeptide that involves in a wide variety of physiological and pathological events, mainly exerts its roles by neurokinin 1 receptor (NK1R), also modulates immune function. However, the roles of SP during immune response to acute bacterial infection of Nile tilapia (Oreochromis niloticus) remain unclear. In this study, the gene of SP precursor (tachykinin precursor 1, TAC1) and the gene of SP receptor (NK1R) from Nile tilapia were identified, and the roles of SP during an acute bacterial infection in a warm water environment were investigated. On-TAC1(Oreochromis niloticus-TAC1) contains conservative SP & NKA peptide sequences and On-NK1R contains seven conservative transmembrane domains. Their transcriptional levels were most abundant in brain and the On-TAC1 transcripts can be induced in the tilapia challenged with Streptococcus agalactiae. Furthermore, the experimental results revealed that On-SP could promote pyroptosis, suppress inflammation, and improve survival rate during acute bacterial infection. The present data lays a theoretical foundation to further elucidate the mechanism of SP protecting fish against pathogens.

Keywords: Acute infection; NK1R; Nile tilapia; Streptococcus agalactiae; Substance P.

MeSH terms

Animals
Cichlids*
Fish Diseases*
Fish Proteins
Streptococcal Infections*
Streptococcus agalactiae / physiology
Substance P

Substances

Fish Proteins
Substance P