Bioprospecting of marine microalgae from Kaohsiung Seacoast for lutein and lipid production

Anil Kumar Patel; Akash Pralhad Vadrale; Yi-Sheng Tseng; Chiu-Wen Chen; Cheng-Di Dong; Reeta Rani Singhania

doi:10.1016/j.biortech.2022.126928

Bioprospecting of marine microalgae from Kaohsiung Seacoast for lutein and lipid production

Bioresour Technol. 2022 May:351:126928. doi: 10.1016/j.biortech.2022.126928. Epub 2022 Mar 4.

Authors

Anil Kumar Patel¹, Akash Pralhad Vadrale², Yi-Sheng Tseng¹, Chiu-Wen Chen³, Cheng-Di Dong⁴, Reeta Rani Singhania¹

Affiliations

¹ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India.
² Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Institute of Aquatic Science and Technology, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
³ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
⁴ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India. Electronic address: cddong@nkust.edu.tw.

PMID: 35257880
DOI: 10.1016/j.biortech.2022.126928

Abstract

A bioprospecting study was conducted from Seawater samples collected at Kaohsiung Seacoast, Taiwan. The current research was aimed to isolate potential lutein-producing strain, evaluate and optimize the best cultivation mode, lutein accumulation stage, lutein-extraction method, and condition to recover maximum lutein (main product) and lipid (byproduct). Biorefinery is the latest approach worldwide to extract multi-products for cost-effectiveness. Selected isolate among several isolates, identified as Chlorella sorokiniana Kh12 and exploited under biorefinery concept for lutein and lipid extraction. Kh12 cultivated under mixotrophy: 2X-(HT)-9k yielded maximum biomass (3.46 g L^-1) and lutein (13.69 mg g^-1) which is among the higher yields reported so far. Among various tested solvents, methanol was the best extractor. Bead milling was most effective to disrupt algal cell walls, seven minutes of milling was best for maximum lutein (7.56 mg g^-1) extraction. Kh12 could be a promising candidate for commercial lutein and lipid co-production based on the outcome.

Keywords: Carotenoids; Chlorella; Lipid; Lutein; Microalgae.

MeSH terms

Biomass
Bioprospecting
Chlorella*
Lipids
Lutein
Microalgae*

Substances

Lipids
Lutein