Single-cell RNA sequencing of mycosis fungoides reveals a cluster of actively proliferating lymphocytes

Australas J Dermatol. 2022 May;63(2):e150-e154. doi: 10.1111/ajd.13794. Epub 2022 Mar 7.

Abstract

A 70-year-old man's chronic erythematous skin lesion in the extremity had developed into a tumour one year before his first visit at our hospital. A biopsy showed atypical lymphocyte-like cells, and immunostaining identified atypical cells as CD3+, CD4+, CD5+ and FOXP3+. Single-cell RNA sequencing (scRNA-seq) analysis using BD Rhapsody revealed the higher expression of CD3, CD4, CD5 and FOXP3 genes in a group of cells that highly expressed genes, such as PCNA, in the S/M phase, which is in agreement with immunofluorescence staining results. The use of scRNA-seq analysis data is expected to promote personalised medicine for cutaneous lymphoma.

Keywords: BD Rhapsody; cutaneous malignant T-cell lymphoma; mycosis fungoides; proliferating cell nuclear antigen; single-cell RNA sequencing.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Forkhead Transcription Factors
  • Humans
  • Lymphocytes / pathology
  • Male
  • Mycosis Fungoides* / genetics
  • Mycosis Fungoides* / pathology
  • Sequence Analysis, RNA
  • Skin Neoplasms* / genetics
  • Skin Neoplasms* / pathology

Substances

  • Forkhead Transcription Factors