Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients

STAR Protoc. 2022 Feb 25;3(1):101204. doi: 10.1016/j.xpro.2022.101204. eCollection 2022 Mar 18.

Abstract

To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMateTM isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.

Keywords: Cell Biology; Cell isolation; Clinical Protocol; Health Sciences; Immunology; Stem Cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD8-Positive T-Lymphocytes
  • Female
  • Freezing
  • Humans
  • Leukocytes*
  • Leukocytes, Mononuclear*
  • Pregnancy
  • T-Lymphocytes, Regulatory