Simultaneous TLC-densitometric analysis of catechin, pyrocatechol and quercetine in gambir block from Pesisir Selatan

Sefrianita Kamal; Meri Susanti; Febriyenti; Erizal Zaini; Dachriyanus Hamidi

doi:10.1016/j.heliyon.2022.e08985

Simultaneous TLC-densitometric analysis of catechin, pyrocatechol and quercetine in gambir block from Pesisir Selatan

Heliyon. 2022 Feb 17;8(3):e08985. doi: 10.1016/j.heliyon.2022.e08985. eCollection 2022 Mar.

Authors

Sefrianita Kamal^{1

2}, Meri Susanti¹, Febriyenti¹, Erizal Zaini¹, Dachriyanus Hamidi¹

Affiliations

¹ Faculty of Pharmacy, Universitas Andalas, Limau Manis, Padang, 25163, West Sumatera, Indonesia.
² Program Studi Farmasi, Universitas Dharma Andalas Padang, West Sumatera, Indonesia.

Abstract

The goal of this study is to use the TLC-densitometric method to determine the concentration of catechin, pyrocatechol, and quercetine in gambir block extracts in a reliable and efficient manner. The best eluent is a mixture of chloroform: ethyl acetate: glacial acetic acid (4:4:2). The concentration of catechin, pyrocatechol and quercetine in gambir block was found to be 25.50 ± 3.13, 0.91 ± 0.60 and 0.83 ± 0.34% (w/w) w/w respectively. The linearity was obtained between 750-2500, 50-350, and 50-350 μg/spot. 12.49-41.63, 0.48-1.60, 3,85-12.83 μg/spots were found to represent the LOD and LOQ, respectively. The proposed approach exhibited great sensitivity, precision, and accuracy, as well as strong linearity.

Keywords: Gambir block; Pesisir Selatan; Simultaneous analysis; TLC densitometry; Uncaria gambir.