Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Farah Kamal; Shulin Shen; Ran Hu; Qianwei Zhang; Nengwen Yin; Yifang Ma; Yuxiang Jiang; Xinfu Xu; Jiana Li; Kun Lu; Cunmin Qu

doi:10.3389/fpls.2022.817419

Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Front Plant Sci. 2022 Feb 16:13:817419. doi: 10.3389/fpls.2022.817419. eCollection 2022.

Authors

Farah Kamal^{1

2}, Shulin Shen^{1

2}, Ran Hu^{1

2}, Qianwei Zhang^{1

2}, Nengwen Yin^{1

2}, Yifang Ma^{1

2}, Yuxiang Jiang^{1

2}, Xinfu Xu^{1

2

3}, Jiana Li^{1

2

3}, Kun Lu^{1

2

3}, Cunmin Qu^{1

2

3}

Affiliations

¹ Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
² Academy of Agricultural Sciences, Southwest University, Chongqing, China.
³ Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, China.

Abstract

Glucosinolates (GSLs) are naturally occurring secondary metabolites found in the Brassicaceae family, which mainly synthesize in the siliques with a wide range of functions. In this study, we investigated the effects of lights on metabolites in siliques of rapeseed through ultra high-performance liquid chromatography (UPLC)-heated electrospray ionization (HESI)-tandem mass spectrometry (MS/MS). A total of 249 metabolites, including 29 phenolic acids, 38 flavonoids, 22 GSLs, 93 uncalculated and 67 unknown compounds, were identified in siliques of rapeseed. Meanwhile, 62 metabolites showed significant differences after shading treatment, which were mainly GSLs and unknown compounds. Interestingly, the amounts of 10 GSLs had high accumulation levels in siliques, while the expression levels of their corresponding biosynthetic genes (AOP, GSL-OH, IGMT, and ST5a) were obviously reduced after shading treatment. Further evidence showed that the amounts of GSLs were significantly reduced in seeds, in accordance with the expression profiles of transporter genes (BnaGTRs). Our findings indicated that lights could affect the accumulation and transportation of GSLs from siliques to seeds in rapeseed. Therefore, this study facilitates a better understanding of metabolic characteristics of siliques and provides insight into the importance of light for GSLs accumulation and transportation in siliques and seeds of rapeseed.

Keywords: Brassica napus L.; glucosinolates; metabolic analysis; shading treatment; siliques.