Lutein production by microalgae using corn starch wastewater pretreated with rapid enzymatic hydrolysis

Heshan Zheng; Yu Wang; Shuo Li; Qinglian Wu; Xiaochi Feng; Yongjie Zheng; Yoong Kit Leong; Duu-Jong Lee; Jo-Shu Chang

doi:10.1016/j.biortech.2022.126940

Lutein production by microalgae using corn starch wastewater pretreated with rapid enzymatic hydrolysis

Bioresour Technol. 2022 May:352:126940. doi: 10.1016/j.biortech.2022.126940. Epub 2022 Mar 1.

Authors

Heshan Zheng¹, Yu Wang¹, Shuo Li², Qinglian Wu³, Xiaochi Feng⁴, Yongjie Zheng¹, Yoong Kit Leong⁵, Duu-Jong Lee⁶, Jo-Shu Chang⁷

Affiliations

¹ College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China.
² College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China; Urban Water Resources Development and Northern National Engineering Research Center, Harbin 150090, China; School of Environment, Harbin Institute of Technology, Harbin 150090, China.
³ School of Environment, Harbin Institute of Technology, Harbin 150090, China.
⁴ School of Civil and Environmental Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China.
⁵ Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan; Research Center for Smart Sustainable Circular Economy, Tunghai University, Taichung 407, Taiwan.
⁶ Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tang, Hong Kong.
⁷ Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan; Research Center for Smart Sustainable Circular Economy, Tunghai University, Taichung 407, Taiwan; Department of Chemical Engineering, National Cheng-Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.

PMID: 35245649
DOI: 10.1016/j.biortech.2022.126940

Abstract

The main purpose of this study was to explore the pretreatment process of corn starch wastewater (CSW) and engineered microalgae cultivation strategy to improve the nutrient recovery from wastewater and the yield of microalgae lutein. One-stage enzymatic hydrolysis utilizing α-amylase and glucoamylase simultaneously was established to efficiently harvest a maximum concentration of reducing sugar content of 7.26 g/L from CSW in 50 min. Lutein yield of 10.96 mg/L was obtained under 24 h continuous illumination with 2200 Lux light intensity. Furthermore, a cyclic feeding cultivation strategy was developed to improve lutein accumulation and COD removal up to 25.9 mg/L and 50.7%, respectively, after three cultivation cycles. Lutein yield of 14.86 mg/L and COD removal efficiency of 73.2% was achieved with further implementation in actual wastewater. This work provided a new perspective in developing the potential of cultivating microalgae with corn starch wastewater to produce high-value lutein.

Keywords: Circular Economy; Enzymatic Pretreatment; Lutein; Microalgae; Starch Wastewater.

MeSH terms

Biomass
Hydrolysis
Lutein
Microalgae*
Starch
Wastewater
Zea mays

Substances

Waste Water
Starch
Lutein