Hormones residues in bovine animals: Sampling, analysis and health risk assessment

Fadl Moussa; Samah Doumiati; Nicola Bernabò; Barbara Barboni; Farouk Jaber; Samia Mokh

doi:10.1016/j.steroids.2022.108994

Hormones residues in bovine animals: Sampling, analysis and health risk assessment

Steroids. 2022 May:181:108994. doi: 10.1016/j.steroids.2022.108994. Epub 2022 Mar 1.

Authors

Fadl Moussa¹, Samah Doumiati², Nicola Bernabò³, Barbara Barboni³, Farouk Jaber⁴, Samia Mokh⁵

Affiliations

¹ Doctoral School of Science and Technology Lebanese University, Beirut, Lebanon; Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Italy.
² Faculty of Public Health- I - Lebanese University, Hadath, Lebanon.
³ Faculty of Bioscience and Technology for Food, Agriculture, and Environment, University of Teramo, Italy.
⁴ National Council for Scientific Research (CNRS), Lebanese Atomic Energy Commission (LAEC), Laboratory for Analysis of Organic Compound (LACO), Beirut, Lebanon; Faculty of Sciences I, Lebanese University, Hadath, Lebanon. Electronic address: farouk-jaber@ul.edu.lb.
⁵ Faculty of Public Health- I - Lebanese University, Hadath, Lebanon; National Council for Scientific Research (CNRS), Lebanese Atomic Energy Commission (LAEC), Laboratory for Analysis of Organic Compound (LACO), Beirut, Lebanon. Electronic address: samia.mokh@ul.edu.lb.

PMID: 35245532
DOI: 10.1016/j.steroids.2022.108994

Abstract

The use of hormones for breeding animal livestock has been banned since 1981 under the Council Directive 81/602/EC. So far, each country should monitor the use of anabolic hormones in animal production to protect the consumer's health against these unwanted residues. This paper presents the research results on steroid and non-steroid hormones residues carried out in Lebanon from 2018 to 2020. Using a newly developed and validated LC-MS/MS method, the detection and the quantification of hormones in bovine matrices were done. The targeted matrices were muscle, liver, kidney, and bile. A total of two-hundred and forty-seven samples were collected from different slaughterhouses located in six different cities in Lebanon. Interestingly, only four hormones were found: testosterone, progesterone, epitestosterone, and 6 propyl 2thiouracil. Based on the obtained data, the estimated daily intake, hazard quotient, and hazard index were calculated to evaluate an exposure assessment.

Keywords: Bovine matrices; Hormones residues; Human consumption; Non-compliant samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Chromatography, Liquid
Hormones*
Progesterone
Risk Assessment
Tandem Mass Spectrometry* / methods
Testosterone

Substances

Hormones
Testosterone
Progesterone